The cloning of cyclin B3 and its gene expression during hormonally induced spermatogenesis in the teleost, Anguilla japonica

Biochemical and Biophysical Research Communications
Hiroko Kajiura-KobayashiYoshitaka Nagahama

Abstract

We cloned cyclin B1, B2, and B3 cDNAs from the eel testis. Northern blot analysis indicated that these cyclin B mRNAs were expressed and increased from day 3 onward after the hormonal induction of spermatogenesis, and that cyclin B3 was most dominantly expressed during spermatogenesis. In situ hybridization showed that cyclin B1 and B2 were present from the spermatogonium stage to the spermatocyte stage. On the other hand, cyclin B3 mRNA was present only in spermatogonia. Although mouse cyclin B3 is expressed specifically in the early meiotic prophase, these results indicate that eel cyclin B3 expression is limited during spermatogenesis to spermatogonia, but is not present in spermatocytes. These facts together suggest that eel cyclin B3 is specifically involved in spermatogonial proliferation (mitosis), but not in meiosis.

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T MiuraY Nagahama
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·J P TassanE A Nigg
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·J LiD J Donoghue
Sep 24, 1999·Developmental Biology·T Kishimoto
Jun 30, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·N YoshidaM Yamashita
Aug 20, 2002·The Journal of Biological Chemistry·Thomas B NguyenAndrew Koff
Jan 14, 2003·Nature Cell Biology·Mark JackmanJonathon Pines

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Citations

May 17, 2008·Development, Growth & Differentiation·Yoshitaka Nagahama, Masakane Yamashita
Mar 4, 2010·Molecular Reproduction and Development·Jean-Claude LozanoGérard Peaucellier
May 26, 2012·Biochemical and Biophysical Research Communications·Satoshi YoshitomeEikichi Hashimoto
Mar 1, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hiroko Kajiura-KobayashiYoshitaka Nagahama

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