The closed eye harbours a unique microbiome in dry eye disease.

Scientific Reports
Kent A WillisC. V. Lal

Abstract

Dry eye affects millions of individuals. In experimental models, dry eye disease is associated with T helper cell 17-mediated inflammation of the ocular surface that may cause persistent damage to the corneal epithelium. However, the initiating and perpetuating factors associated with chronic inflammation of the ocular surface remain unclear. The ocular microbiota alters ocular surface inflammation and may influence dry eye disease development and progression. Here, we collected serial samples of tears on awakening from sleep, closed eye tears, during a randomized clinical trial of a non-pharmaceutical dry eye therapy and used 16S rRNA metabarcoding to characterize the microbiome. We show the closed dry eye microbiome is distinct from the healthy closed eye microbiome, and that the microbiome remains distinct despite daily saline eye wash upon awakening. The ocular microbiome was described only recently, and this report implicates a distinct microbiome in ocular disease development. Our findings suggest an interplay between microbial commensals and inflammation on the ocular surface. This information may inform future studies of the pathophysiological mechanisms of dry eye disease.

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Citations

Feb 8, 2021·Experimental Eye Research·Konduri RanjithSisinthy Shivaji
Feb 17, 2021·Current Eye Research·Philippe DaullJean-Sébastien Garrigue
Feb 24, 2021·The Ocular Surface·Jeremias G Galletti, Cintia S de Paiva
Jun 5, 2021·Frontiers in Pharmacology·Brynn A HollingsworthLanyn P Taliaferro
Sep 16, 2021·Investigative Ophthalmology & Visual Science·Zhang ZhangHaidong Zou

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Methods Mentioned

BETA
PCR

Clinical Trials Mentioned

NCT03332342

Software Mentioned

REDCap
Micropower
Calypso
PERMANOVA

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