The cme gene of Clostridium difficile confers multidrug resistance in Enterococcus faecalis

FEMS Microbiology Letters
Sacha LebelThierry Lambert

Abstract

Antibiotic resistance in C. difficile by efflux has been previously suggested. The genome of C. difficile 630 was screened for sequences encoding putative proteins homologous to NorA from Staphylococcus aureus. Four ORFs homologous to efflux genes were cloned into the pAT79 shuttle vector under the control of transcription and translation signals of Gram-positive bacteria and expressed in Enterococcus faecalis JH2-2 and S. aureus RN4220. One of these sequences, designated cme conferred resistance to ethidium bromide, safranin O, and erythromycin in E. faecalis. The three other ORFs did not confer detectable resistance in both bacteria.

Citations

Aug 15, 2009·Drugs·Xian-Zhi Li, Hiroshi Nikaido
Aug 11, 2010·Antimicrobial Agents and Chemotherapy·Sébastien CoyneBruno Périchon
Oct 5, 2012·Clinical Microbiology Reviews·Lucía Fernández, Robert E W Hancock
May 25, 2007·Aging Cell·Ramachander V N TuragaMichel Lebel
Jan 1, 2013·International Journal of Bacteriology·Sanath KumarManuel F Varela
May 26, 2005·The Journal of Antimicrobial Chemotherapy·Keith Poole

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