The cohesin-like RecN protein stimulates RecA-mediated recombinational repair of DNA double-strand breaks

Nature Communications
Lee A UrangaShelley L Lusetti

Abstract

RecN is a cohesin-like protein involved in DNA double-strand break repair in bacteria. The RecA recombinase functions to mediate repair via homologous DNA strand invasion to form D-loops. Here we provide evidence that the RecN protein stimulates the DNA strand invasion step of RecA-mediated recombinational DNA repair. The intermolecular DNA tethering activity of RecN protein described previously cannot fully explain this novel activity since stimulation of RecA function is species-specific and requires RecN ATP hydrolysis. Further, DNA-bound RecA protein increases the rate of ATP hydrolysis catalysed by RecN during the DNA pairing reaction. DNA-dependent RecN ATPase kinetics are affected by RecA protein in a manner suggesting a specific order of protein-DNA assembly, with RecN acting after RecA binds DNA. We present a model for RecN function that includes presynaptic stimulation of the bacterial repair pathway perhaps by contributing to the RecA homology search before ternary complex formation.

References

Jan 1, 1984·Molecular & General Genetics : MGG·S M PicksleyR G Lloyd
Dec 28, 1983·Biochemical and Biophysical Research Communications·S J GargerL K Grill
May 17, 1984·Nature·P Howard-FlandersA Stasiak
Jan 1, 1983·Molecular & General Genetics : MGG·R G LloydC Prescott
Nov 13, 1998·The Journal of Biological Chemistry·K Adzuma
Jun 5, 2002·Annual Review of Biochemistry·Shelley L Lusetti, Michael M Cox
Jun 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jong-Il Kim, Michael M Cox
Feb 25, 2003·The Journal of Biological Chemistry·Shelley L LusettiMichael M Cox
Jan 1, 2004·The Journal of Biological Chemistry·Mara E RobuMichael M Cox
Jan 14, 2004·BMC Microbiology·Julie Malia EggingtonMichael Matthew Cox
Oct 7, 2004·The Journal of Biological Chemistry·Olga M Mazina, Alexander V Mazin
Dec 31, 2005·Journal of Bacteriology·Humberto SanchezJuan C Alonso
Apr 25, 2006·Nature Reviews. Molecular Cell Biology·Tatsuya Hirano
Aug 18, 2006·The Journal of Biological Chemistry·Kohji NagashimaTakashi Hishida
Nov 15, 2007·Nucleic Acids Research·Humberto SanchezJuan C Alonso
Jan 2, 2008·Cell Research·Xuan Li, Wolf-Dietrich Heyer
Feb 23, 2008·The Journal of Biological Chemistry·YoungHo KwonPatrick Sung
Apr 3, 2010·The Journal of Biological Chemistry·Emigdio D ReyesShelley L Lusetti
Nov 30, 2010·Biophysical Journal·Roberto J PezzaPiero R Bianco
Feb 16, 2011·Journal of Cell Science·Maria T Ocampo-Hafalla, Frank Uhlmann
Apr 27, 2011·FEMS Microbiology Reviews·Silvia AyoraJuan C Alonso
Feb 10, 2012·Nature·Anthony L Forget, Stephen C Kowalczykowski
Feb 14, 2012·Journal of Cell Science·P M KrawczykJ A Aten
Apr 10, 2012·Nature Cell Biology·Judith Miné-Hattab, Rodney Rothstein
Dec 15, 2012·ELife·Kaushik RagunathanTaekjip Ha
Dec 15, 2012·ELife·Bryan Gibb, Eric C Greene
Dec 25, 2012·Current Opinion in Structural Biology·Maria Spies
Feb 6, 2013·DNA Repair·Juan C AlonsoKunio Takeyasu
Jun 27, 2013·The Journal of Biological Chemistry·Sergio R Santa MariaHannah L Klein
Aug 27, 2013·The Journal of Biological Chemistry·Kenji KeyamuraTakashi Hishida
Dec 24, 2013·Nature·Christian LesterlinDavid J Sherratt
Feb 4, 2014·Molecular Cell·William Douglass Wright, Wolf-Dietrich Heyer
Mar 13, 2014·Microbiology·Ingvild Odsbu, Kirsten Skarstad
May 16, 2014·Nature Reviews. Molecular Cell Biology·Jörg RenkawitzStefan Jentsch

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Citations

Nov 14, 2018·Molecular Microbiology·Alyson R WarrSteven J Sandler
Mar 10, 2020·PLoS Genetics·Laasya SamhitaDeepa Agashe
Nov 8, 2018·Scientific Reports·Thomas C RöschPeter L Graumann
Jul 31, 2019·Scientific Reports·Deepika PrasadK Muniyappa
Mar 14, 2020·International Journal of Molecular Sciences·Daniela GonzálezGloria Levicán
Jun 17, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ekaterina ZabolotnayaRobert M Henderson
Aug 21, 2020·Genetics·Anastasiia N Klimova, Steven J Sandler
May 7, 2021·Genetics·Anastasiia N Klimova, Steven J Sandler
Jun 6, 2021·Trends in Genetics : TIG·Steven T Bruckbauer, Michael M Cox
Jun 15, 2021·Frontiers in Microbiology·Paloma LaraKaty Juárez
Jun 19, 2021·Frontiers in Microbiology·Inoka P MenikpuragePaola E Mera
Aug 3, 2021·Journal of Bacteriology·Emma K McLeanLyle A Simmons

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Methods Mentioned

BETA
X-ray
pull-down
electrophoresis

Software Mentioned

Analyst PC Image
Foto

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