The cold-induced basic helix-loop-helix transcription factor gene MdCIbHLH1 encodes an ICE-like protein in apple.

BMC Plant Biology
Xiao-Ming FengYu-Jin Hao

Abstract

Plant growth is greatly affected by low temperatures, and the expression of a number of genes is induced by cold stress. Although many genes in the cold signaling pathway have been identified in Arabidopsis, little is known about the transcription factors involved in the cold stress response in apple. Here, we show that the apple bHLH (basic helix-loop-helix) gene MdCIbHLH1 (Cold-Induced bHLH1), which encodes an ICE-like protein, was noticeably induced in response to cold stress. The MdCIbHLH1 protein specifically bound to the MYC recognition sequences in the AtCBF3 promoter, and MdCIbHLH1 overexpression enhanced cold tolerance in transgenic Arabidopsis. In addition, the MdCIbHLH1 protein bound to the promoters of MdCBF2 and favorably contributed to cold tolerance in transgenic apple plants by upregulating the expression of MdCBF2 through the CBF (C-repeat-binding factor) pathway. Our findings indicate that MdCIbHLH1 functions in stress tolerance in different species. For example, ectopic MdCIbHLH1 expression conferred enhanced chilling tolerance in transgenic tobacco. Finally, we observed that cold induces the degradation of the MdCIbHLH1 protein in apple and that this degradation was potentially mediated by ubiquitination and...Continue Reading

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Citations

Apr 18, 2013·Biochemistry. Biokhimii︠a︡·Yue JiangZhi-Fu Guo
May 7, 2016·Journal of Plant Physiology·Rui-Fen ZhangChun-Xiang You
Nov 13, 2016·Plant Physiology and Biochemistry : PPB·Jinhua XuXilin Hou
Feb 13, 2019·Plant Molecular Biology·Jianzhao LiSongling Bai
Jul 15, 2020·Plant Signaling & Behavior·Pengfei DiaoNana Ma
Apr 20, 2017·The Plant Journal : for Cell and Molecular Biology·Da-Gang HuYu-Jin Hao
Sep 8, 2017·Frontiers in Plant Science·Hong-Mei YuanXi Huang
Feb 25, 2019·Molecular Biology Reports·Marina L DíazAlicia Carrera
Mar 3, 2020·Frontiers in Plant Science·Evangelos KaragiannisAthanassios Molassiotis
Sep 15, 2020·Plant Signaling & Behavior·Rakesh Kumar VermaViswanathan Chinnusamy
Oct 9, 2018·Frontiers in Plant Science·Michael WisniewskiRajeev Arora
Sep 5, 2018·Plant Physiology and Biochemistry : PPB·Xinran ChengYan Xiang
Jan 27, 2021·The Plant Journal : for Cell and Molecular Biology·Yicheng WangXuesen Chen

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Datasets Mentioned

BETA
ABS50521
ABF48720

Methods Mentioned

BETA
transgenic
ChIP-PCR
immunoprecipitation
ICE
PCR
electrophoresis
MDA
ubiquitination
sumoylation
ChIP

Software Mentioned

SAS ( Statistical Analysis System )
BLAST
MEGA

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