The combination of isoflurane and caspase 8 inhibition results in sustained neuroprotection in rats subject to focal cerebral ischemia

Anesthesia and Analgesia
Satoki InoueP M Patel

Abstract

Although isoflurane can reduce ischemic neuronal injury after short postischemic recovery intervals, data from our laboratory have demonstrated that this neuroprotection is not sustained and that delayed apoptotic neuronal death, mediated in part by activation of caspases, contributes to the gradual increase in the size of the infarction. We tested the hypothesis that the neuroprotective efficacy of isoflurane can be prolonged with the administration of z-IETD-fmk, a specific inhibitor of caspase 8. Fasted Wister rats were anesthetized with isoflurane and randomly allocated to awake-vehicle, isoflurane-vehicle, awake-IETD, or isoflurane-IETD groups (n = 25 per group). Animals were subjected to 60 min focal ischemia by filament occlusion of the middle cerebral artery (MCAO). Daily intracerebroventricular injections of z-IETD-fmk or vehicle were administered via an implanted cannula starting before ischemia and continuing until 14 days post-MCAO. Neurological assessment was performed 14 days after ischemia after which the volume of cerebral infarction and number of intact neurons in the peri-infarct cortex were determined. Total infarction volume was less in the isoflurane-IETD group than in awake-vehicle, isoflurane-vehicle, and...Continue Reading

References

Jul 1, 1978·The Journal of Physiology·J L Barker, B R Ransom
Mar 1, 1990·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R A SwansonF R Sharp
Jan 1, 1991·Annals of the New York Academy of Sciences·J Yang, C F Zorumski
Sep 1, 1990·British Journal of Pharmacology·E Puil, H el-Beheiry
Jan 1, 1989·Stroke; a Journal of Cerebral Circulation·E Z LongaR Cummins
Jul 1, 1995·Stroke; a Journal of Cerebral Circulation·Y LiC Zaloga
May 1, 1995·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Y LiC Zaloga
Mar 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·C DuD W Choi
Mar 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·T BackB D Watson
Sep 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S A Loddick, N J Rothwell
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·H HaraM A Moskowitz
Apr 1, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H HaraM A Moskowitz
Aug 1, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R SaitoW D Heiss
Mar 14, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M EndresM A Moskowitz
Jul 1, 1996·Nature Biotechnology·R Hoyle
Jul 4, 1998·Brain Research. Brain Research Reviews·S NakanishiM Okada
Sep 28, 1998·The European Journal of Neuroscience·T HimiS Murota
Nov 26, 1998·The Journal of Biological Chemistry·M Garcia-CalvoN A Thornberry
Jan 23, 1999·Anesthesia and Analgesia·V Soonthon-BrantM Watson
Aug 11, 1999·Trends in Neurosciences·U DirnaglM A Moskowitz
Jan 8, 2000·Stroke; a Journal of Cerebral Circulation·H LiC Iadecola
Feb 15, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S H Graham, J Chen
Oct 6, 2001·Stroke; a Journal of Cerebral Circulation·Y Morita-FujimuraP H Chan
Nov 3, 2001·Brain Research. Brain Research Reviews·M Dubois-DauphinA Savioz
Dec 12, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K JinD A Greenberg
Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·N PlesnilaM A Moskowitz
Dec 26, 2001·Anesthesiology·Breandan L SullivanPhilip E Bickler
Apr 26, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Guodong CaoJun Chen

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Citations

Dec 11, 2007·Molecular Neurobiology·Venkata Prasuja NakkaRam Raghubir
Feb 7, 2013·The Journal of Biological Chemistry·Michael FrickerGuy C Brown
Dec 22, 2010·British Journal of Anaesthesia·K M Erickson, D J Cole
Nov 10, 2009·Injury·Nasser A TawfeeqWa'el S Taha
Mar 21, 2009·Neurological Research·Gerald A MatchettJohn H Zhang
Aug 19, 2016·Translational Stroke Research·Ulrike HoffmannDavid S Warner
Nov 24, 2011·Korean journal of anesthesiology·Hee-Pyoung ParkYoung-Tae Jeon
Nov 6, 2015·Cell Death & Disease·A P ShabanzadehP D Koeberle
Sep 9, 2006·Current Opinion in Anaesthesiology·Ines P Koerner, Ansgar M Brambrink
Jan 2, 2007·Anesthesiology·Hiroaki SakaiDavid S Warner
Mar 26, 2008·Journal of Neurosurgical Anesthesiology·Jeffrey J Pasternak, William L Lanier
Sep 18, 2007·Current Opinion in Anaesthesiology·Brian P Head, Piyush Patel
Oct 12, 2010·Critical Reviews in Biochemistry and Molecular Biology·Sara BaratchiJagat R Kanwar
Mar 20, 2009·Journal of Neurosurgical Anesthesiology·Jeffrey J Pasternak, William L Lanier
Dec 17, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jun Li, Louise D McCullough

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