The combination of mannitol and temozolomide increases the effectiveness of stem cell treatment in a chronic stroke model

Cytotherapy
Chunggab ChoiOk Joon Kim

Abstract

The blood-brain barrier (BBB) presents a significant challenge to the therapeutic efficacy of stem cells in chronic stroke. Various methods have been developed to increase BBB permeability, but these are associated with adverse effects and are, therefore, not clinically applicable. We recently identified that combination drug treatment of mannitol and temozolomide improved BBB permeability in vitro. Here, we investigated whether this combination could increase the effectiveness of stem cell treatment in an animal model of chronic ischemic stroke. Chronic stroke was induced in rats by middle cerebral artery occlusion (MCAo). After then, rats were administered human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs) by intravenous injection with or without combination drug treatment of mannitol and temozolomide. To evaluate the therapeutic efficacy, behavioral and immunohistochemical tests were performed, and the differences among control, stem cell only, combination drug only and stem cell with combination drug treatment were analyzed. Although no hUC-MSCs were detected in any group, treatment with stem cells and combination drug of mannitol and temozolomide increased the intracerebral delivery of hCD63-positive microve...Continue Reading

Citations

Sep 10, 2020·Tissue Engineering and Regenerative Medicine·Sang-Hoon LeeDong-Hyuk Park
Jan 4, 2019·Journal of Biological Engineering·Shirin SaberianpourMohammad Nouri
Oct 25, 2020·International Journal of Molecular Sciences·Sanghun LeeNam Keun Kim
Oct 16, 2020·Journal of Stroke·Shuai ZhangXiaofeng Jia
Jul 6, 2021·Stem Cells International·Yinghan GuoGao Chen

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