The combination of Panax ginseng and Angelica sinensis alleviates ischemia brain injury by suppressing NLRP3 inflammasome activation and microglial pyroptosis

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Jia HuHongmei Tan

Abstract

The combination of Panax ginseng and Angelica sinensis (CPA) has been used to treat stroke for one thousand years and demonstrated clinically to have satisfied effects. However, the underlying mechanism remains unknown. We investigate whether CPA has neuroprotective effects via suppressing Nod-like receptor protein 3 (NLRP3) inflammasome and microglial pyroptosis against ischemic injury in transient middle cerebral artery occlusion (MCAO) rats. Male rats were divided randomly into sham operated, MCAO, MCC950 (NLRP3-specific inhibitor) and CPA groups. Neurological deficits, glucose uptake, infarct size, activation of NLRP3 inflammasomes, microglial pyroptosis and related signaling pathways were detected. BV-2 microglial cells subjected to oxygen-glucose deprivation/reoxygenation (OGD/R) were used in in vitro experiments. Compared with sham rats, elevated level of proinflammatory interleukin-1β (IL-1β) in plasma, neurological function deficit, reduced glucose uptake in ipsilateral hemisphere, obvious infarct size, the activation of NLRP3 inflammasomes and enhanced microglial pyroptosis were presented in MCAO rats. The administrations of MCC950 and CPA respectively reversed the results. In vitro OGD/R induced the release of lactat...Continue Reading

Citations

Nov 24, 2020·International Immunopharmacology·Xin WangQingjiang Jin
Dec 10, 2020·Journal of Immunology Research·Lanqing MengLingzhang Meng
Jun 29, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Zirong PanGuanhua Du
Aug 8, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yan HanYong-Hua Zhao

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