The Comet Assay and its applications in the field of ecotoxicology: a mature tool that continues to expand its perspectives

Frontiers in Genetics
Joaquín de LapuenteMário Pacheco

Abstract

Since Singh and colleagues, in 1988, launched to the scientific community the alkaline Single Cell Gel Electrophoresis (SCGE) protocol, or Comet Assay, its uses and applications has been increasing. The thematic areas of its current employment in the evaluation of genetic toxicity are vast, either in vitro or in vivo, both in the laboratory and in the environment, terrestrial or aquatic. It has been applied to a wide range of experimental models: bacteria, fungi, cells culture, arthropods, fishes, amphibians, reptiles, mammals, and humans. This document is intended to be a comprehensive review of what has been published to date on the field of ecotoxicology, aiming at the following main aspects: (i) to show the most relevant experimental models used as bioindicators both in the laboratory and in the field. Fishes are clearly the most adopted group, reflecting their popularity as bioindicator models, as well as a primary concern over the aquatic environment health. Amphibians are among the most sensitive organisms to environmental changes, mainly due to an early aquatic-dependent development stage and a highly permeable skin. Moreover, in the terrestrial approach, earthworms, plants or mammalians are excellent organisms to be us...Continue Reading

References

Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Jan 1, 1995·Bulletin of Environmental Contamination and Toxicology·L Verschaeve, J Gilles
Jan 1, 1995·Environmental and Molecular Mutagenesis·R PandrangiM Vrzoc
Jan 1, 1996·Environmental and Molecular Mutagenesis·S RalphM Vrzoc
Jan 5, 2000·Environmental and Molecular Mutagenesis·S Cotelle, J F Férard
Jan 5, 2002·Environmental and Molecular Mutagenesis·P RajaguruK Kalaiselvi
Dec 31, 2002·Mutation Research·Alisson Marques de Miranda Cabral GontijoDaisy Maria Favero Salvadori
Nov 6, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D CavalloS Iavicoli
Feb 13, 2004·Marine Biotechnology·R B WalterC A Walter
Mar 24, 2004·Mutation Research·Nikolai K ChemerisVyacheslav A Logunov
Apr 20, 2005·Reviews on Environmental Health·Te-Hsiu MaElizabeth Owens
Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·Daniela MauriciPhillipe Vanparys
Aug 1, 2006·Environmental and Molecular Mutagenesis·Stanley O AniaguMatthew J Winter

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Citations

Dec 18, 2015·Aquatic Toxicology·Francine GirardelloJoão Antonio Pêgas Henriques
Sep 1, 2015·Frontiers in Genetics·Sabine A S LangieAndrew R Collins
Jun 28, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Niti SharmaRupam Sharma
Sep 11, 2016·Environmental Pollution·Dragana MatićBranka Vuković Gačić
Aug 24, 2017·The Journal of Eukaryotic Microbiology·Barbara KammerlanderBettina Sonntag
Aug 25, 2017·Journal of Toxicology and Environmental Health. Part a·Andrea SahlmannKetil Hylland
Jul 26, 2019·Environmental Toxicology and Chemistry·Andrea SahlmannKetil Hylland
Nov 2, 2019·The Journal of Experimental Biology·Jan LubawyHervé Colinet
Jul 6, 2016·Archives of Toxicology·M GleiW Schlörmann
Apr 9, 2020·Environmental Toxicology and Chemistry·Alexis M WormingtonChristopher J Martyniuk
Oct 6, 2020·Frontiers in Microbiology·Costantino VischettiEdoardo Puglisi
Nov 30, 2018·Forensic Sciences Research·Ritesh Kumar Shukla
Dec 11, 2019·Toxicology and Industrial Health·Dalia Abdel Moneim KheirallahNoura Abdel Haleem Toto
Jul 28, 2019·The Science of the Total Environment·Gerardo Heredia-GarcíaOctavio Dublán-García
Mar 27, 2020·Journal of Hazardous Materials·Yuanbo LiZhenjun Sun
Mar 7, 2021·Methods and Protocols·Maria Rita FabbriziJason L Parsons
Feb 23, 2021·Toxicology Research·Eugenia CordelliFrancesca Pacchierotti
Aug 15, 2017·Marine Environmental Research·Jonny BeyerMerete Schøyen
May 21, 2019·Ecotoxicology and Environmental Safety·Donaji J González-MilleRogelio Flores-Ramírez
Jun 29, 2021·The Science of the Total Environment·Patrícia SaikiThiago Lopes Rocha
Aug 27, 2021·Metabolites·Blandine TramuntWalter Wahli

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Methods Mentioned

BETA
Electrophoresis
Assay

Software Mentioned

Comet Assay

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