The comet assay in higher terrestrial plant model: Review and evolutionary trends

Environmental Pollution
Caroline LanierAnnabelle Deram

Abstract

The comet assay is a sensitive technique for the measurement of DNA damage in individual cells. Although it has been primarily applied to animal cells, its adaptation to higher plant tissues significantly extends the utility of plants for environmental genotoxicity research. The present review focuses on 101 key publications and discusses protocols and evolutionary trends specific to higher plants. General consensus validates the use of the percentage of DNA found in the tail, the alkaline version of the test and root study. The comet protocol has proved its effectiveness and its adaptability for cultivated plant models. Its transposition in wild plants thus appears as a logical evolution. However, certain aspects of the protocol can be improved, namely through the systematic use of positive controls and increasing the number of nuclei read. These optimizations will permit the increase in the performance of this test, namely when interpreting mechanistic and physiological phenomena.

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Citations

Aug 2, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Kamal K PandaBrahma B Panda
Jul 6, 2016·Archives of Toxicology·M GleiW Schlörmann
Aug 31, 2016·Frontiers in Plant Science·Elena KuzminskyGiuseppe Scarascia-Mugnozza
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Mar 17, 2018·Environmental Science and Pollution Research International·Fabien BernardDamien Cuny
Jul 13, 2018·Frontiers in Plant Science·Nicol Caplin, Neil Willey
Jan 1, 2017·Bulletin of Environmental Contamination and Toxicology·Veerapas Na Roi-EtChart Chiemchaisri
Jul 16, 2020·Environmental Science and Pollution Research International·Nafiseh MansouriAkbar Hassanzadeh
Oct 20, 2020·Environmental Toxicology and Chemistry·Anithadevi Kenday SivaramMallavarapu Megharaj
Apr 14, 2020·Ecotoxicology and Environmental Safety·Xue TianWenjie Yang

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