The common bile duct ligation in rat: A relevant in vivo model to study the role of mechanical stress on cell and matrix behaviour.

Histochemistry and Cell Biology
Christelle GuyotAlexis Desmoulière

Abstract

Common bile duct ligation leads to bile accumulation and liver fibrosis. In this model, little attention has been dedicated to the modification of the common bile duct. We have studied by histochemistry and immunohistochemistry, 3 and 5 days after ligation, the connective tissue modifications of the common bile duct wall. After bile duct ligation, compared with normal bile duct, a strong increase of the bile duct diameter, due to bile stasis, and a thickness of the bile duct wall were observed; numerous myofibroblasts expressing alpha-smooth muscle actin appeared in parallel with the detection of many proliferating connective tissue cells. These myofibroblasts secreted very early high amount of elastic fibre components, elastin and fibrillin-1. Elastic fibre increase was also observed close to the epithelial cell layer. Procollagen type III deposition was also induced 3 days after ligation but decreased thereafter, underlining that myofibroblasts modify their synthesis of extracellular matrix components to comply with the request. We show here that common bile duct ligation represents an invaluable model to study myofibroblastic differentiation and extracellular matrix adaptation produced by an acute mechanical stress.

References

Jun 8, 2001·Journal of Hepatology·L DubuissonA Desmoulière
Apr 30, 2002·The American Journal of Surgical Pathology·Thierry LamireauAlexis Desmoulière
May 4, 2002·Nature Reviews. Molecular Cell Biology·James J TomasekRobert A Brown
Jun 18, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Valerie ParadisPierre Bedossa
Oct 29, 2003·Current Opinion in Biotechnology·Boris Hinz, Giulio Gabbiani
Dec 4, 2003·Thrombosis and Haemostasis·Boris Hinz, Giulio Gabbiani
Dec 9, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Dionne LorenaAlexis Desmoulière
Dec 24, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Alexis DesmoulièreGiulio Gabbiani

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Citations

Dec 7, 2006·Histochemistry and Cell Biology·Douglas J TaatjesJürgen Roth
Nov 1, 2007·Histochemistry and Cell Biology·Christian ZuberJürgen Roth
Sep 8, 2010·Journal of Feline Medicine and Surgery·Robert W Furneaux
Aug 23, 2008·Journal of Gastroenterology and Hepatology·Pavel TomsikStanislav Micuda
Mar 27, 2013·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Simone Nunes de CarvalhoLais de Carvalho
Aug 30, 2007·Critical Reviews in Therapeutic Drug Carrier Systems·Kun Cheng, Ram I Mahato
Jan 20, 2015·Current Pathobiology Reports·Rebecca G Wells
Mar 1, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rebecca G Wells
Jan 10, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Antonella PellicoroJohn P Iredale
Feb 26, 2013·Biochimica Et Biophysica Acta·Rebecca G Wells
Nov 9, 2016·Frontiers in Physiology·Jiří Kanta
Jul 5, 2016·World Journal of Methodology·Marlein Miranda ConaYicheng Ni
Jul 13, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Natalia Nieto

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