The common inhalational anesthetic isoflurane induces apoptosis via activation of inositol 1,4,5-trisphosphate receptors.

Anesthesiology
Huafeng WeiRoderic G Eckenhoff

Abstract

Isoflurane induces cell apoptosis by an unknown mechanism. The authors hypothesized that isoflurane activates inositol 1,4,5-trisphosphate (IP3) receptors on the endoplasmic reticulum (ER) membrane, causing excessive calcium release, triggering apoptosis. The authors determined isoflurane-induced cytotoxicity by measuring caspase-3 activity, lactate dehydrogenase release, MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) reduction, and imaging analysis of cell damage markers (annexin V and propidium iodide staining) in different cell types. The authors used the chicken B lymphocyte with a total knock-out of IP3 receptors, PC12 cells with elevated IP3 receptor activity (transfected with L286V presenilin 1), striatal cells with a knock-in of Q111 Huntingtin, and each cell line's corresponding wild-type controls. The authors also measured the isoflurane-evoked changes of calcium concentration in cytosol and/or mitochondria in these cells. Isoflurane induced apoptosis concentration- and time-dependently, and sequentially elevated cytosolic and then mitochondrial calcium in the chicken B-lymphocyte wild-type but not the IP3 receptor total knock-out cells. Thapsigargin, a calciu...Continue Reading

References

Jan 28, 1993·Nature·M J Berridge
Jun 1, 1997·Molecular and Cellular Biology·T Jayaraman, A R Marks
Nov 25, 2000·Human Molecular Genetics·F TrettelM E MacDonald
Jul 10, 2001·Neurobiology of Disease·M A LeissringI Parker
Oct 11, 2002·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Rie Kato, Pierre Foëx
Nov 5, 2002·Nature Reviews. Neuroscience·Frank M LaFerla
Jan 25, 2003·Cell Calcium·C CamelloA V Tepikin
Mar 26, 2003·Anesthesia and Analgesia·Deborah J CulleyGregory Crosby
Dec 17, 2003·Journal of Neurosurgical Anesthesiology·David S Warner
Aug 27, 2004·Anesthesiology·Roderic G EckenhoffMaryellen F Eckenhoff
Sep 15, 2004·Journal of Chemical Neuroanatomy·Taiichi KatayamaMasaya Tohyama
Nov 9, 2004·Current Biology : CB·C Jane HansonH Llewelyn Roderick
Feb 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tie-Shan TangIlya Bezprozvanny
Mar 5, 2005·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Grace E Stutzmann
Mar 22, 2005·Brain Research·Huafeng WeiRoderic G Eckenhoff
Sep 16, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Santanu Banerjee, Gaiti Hasan
Sep 28, 2005·The Journal of Cell Biology·Muniswamy MadeshAron B Fisher
Oct 1, 2005·Science·Akira FutatsugiKatsuhiko Mikoshiba
Oct 4, 2005·The Journal of Clinical Investigation·Chunyan XuJohn C Reed
Jan 5, 2006·JAMA : the Journal of the American Medical Association·Thomas W Sedlak, Solomon H Snyder
Jan 7, 2006·Cell Death and Differentiation·M Boyce, J Yuan
Jan 7, 2006·Cell Death and Differentiation·D LindholmL Korhonen
Aug 16, 2006·Neuro-degenerative Diseases·Zhongcong XieRudolph E Tanzi
Jan 20, 2007·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Zhongcong XieRudolph E Tanzi

❮ Previous
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Citations

Dec 17, 2011·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yilin ZhaoAilin Luo
Dec 22, 2010·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Edward A BittnerZhongcong Xie
Jun 16, 2009·Neurotoxicity Research·Sven BerckerThoralf Kerner
Dec 17, 2009·The Journal of Biological Chemistry·Yiying ZhangZhongcong Xie
May 28, 2011·British Journal of Anaesthesia·A E Hudson, H C Hemmings
May 7, 2010·Cerebral Cortex·Antonia ReimerAndrej Kral
Mar 29, 2011·Cold Spring Harbor Perspectives in Biology·Djalila MekahliLudwig Missiaen
Jan 21, 2010·The Journal of Pharmacology and Experimental Therapeutics·Yifan ZhaoHuafeng Wei
Dec 18, 2009·Pediatric Research·Shouping WangHuafeng Wei
Apr 18, 2009·Anesthesia and Analgesia·Dmitri BaranovUNKNOWN First International Workshop on Anesthetics and Alzheimer's Disease
Oct 13, 2009·Anesthesia and Analgesia·Junxia TangRoderic G Eckenhoff
Sep 16, 2010·Anesthesia and Analgesia·Jennifer E FugateAlejandro A Rabinstein
Sep 24, 2010·Anesthesia and Analgesia·Saadet Inan, Huafeng Wei
Oct 25, 2011·Anesthesia and Analgesia·Huafeng Wei
Apr 25, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Guohua ZhangZhongcong Xie
Jan 5, 2014·British Journal of Anaesthesia·M R NadelsonM S Avidan
Jun 1, 2013·Progress in Neuro-psychopharmacology & Biological Psychiatry·Huafeng Wei, Saadet Inan
Jan 10, 2012·Acta Anaesthesiologica Taiwanica : Official Journal of the Taiwan Society of Anesthesiologists·Rele Ologunde, Daqing Ma
Aug 21, 2012·Anesthesiology Clinics·Ansgar M BrambrinkJeffrey R Kirsch
Dec 30, 2011·Annales Françaises D'anesthèsie Et De Rèanimation·S DahmaniV Laudenbach
Jul 13, 2011·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Junxia X TangMaryellen F Eckenhoff
Dec 3, 2010·Annales Françaises D'anesthèsie Et De Rèanimation·D AndréF Sztark
Jun 12, 2010·Annales Françaises D'anesthèsie Et De Rèanimation·F MonsV Laudenbach
Nov 8, 2015·American Journal of Obstetrics and Gynecology·Olutoyin A OlutoyeOluyinka O Olutoye
Jan 14, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Changlian ZhuKlas Blomgren
Sep 11, 2008·Medical Hypotheses·Qiang XiangAilin Luo
Feb 9, 2011·Journal of Cellular Physiology·Karol OndriasOlga Krizanova

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis