The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles

Stem Cells International
Selin YildirimTunc Akkoc

Abstract

Aim. To compare the effects of various mesenchymal stem cells, those isolated from human exfoliated deciduous teeth (SHEDs), dental pulp stem cells (DPSCs), and dental follicle stem cells (DFSCs), on human peripheral blood mononuclear cells (PBMCs). Method. Mesenchymal stem cells were isolated from three sources in the orofacial region. Characterization and PCR analyses were performed. Lymphocytes were isolated from healthy peripheral venous blood. Lymphocytes were cocultured with stem cells in the presence and absence of IFN-γ and stimulated with anti-CD2, anti-CD3, and anti-CD28 for 3 days. Then, lymphocyte proliferation, the number of CD4(+)FoxP3(+) T regulatory cells, and the levels of Fas/Fas ligand, IL-4, IL-10, and IFN-γ in the culture supernatant were measured. Results. The DFSCs exhibited an enhanced differentiation capacity and an increased number of CD4(+)FoxP3(+) T lymphocytes and suppressed the proliferation and apoptosis of PBMCs compared with SHEDs and DPSCs. The addition of IFN-γ augmented the proliferation of DFSCs. Furthermore, the DFSCs suppressed IL-4 and IFN-γ cytokine levels and enhanced IL-10 levels compared with the other cell sources. Conclusion. These results suggest that IFN-γ stimulates DFSCs by indu...Continue Reading

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Citations

Oct 1, 2016·Stem Cells International·Jessica RatajczakPetra Hilkens
Mar 3, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D GençT Akkoç
Apr 11, 2019·Stem Cells and Development·Jie ZhangChunmiao Jiang
Jan 5, 2019·Current Stem Cell Research & Therapy·Tatjana KanjevacDarko Bosnakovski
Oct 28, 2019·Current Stem Cell Research & Therapy·Chih-Sheng KoWen-Ta Su
Jul 30, 2020·Stem Cells International·Mengyuan WangHongzhi Fang
Aug 30, 2020·International Journal of Molecular Sciences·Anna Labedz-MaslowskaEwa Zuba-Surma
Oct 23, 2019·Stem Cells International·Tao ZhouXin Zhou
Jun 11, 2019·Tissue Engineering. Part B, Reviews·Karim M Fawzy El-SayedChristof E Dörfer
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Dixin CuiMian Wan
Dec 7, 2021·Frontiers in Immunology·Bo YangYan Wang

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Methods Mentioned

BETA
flow cytometry
PCR

Software Mentioned

LightCycler
SPSS
GraphPad Prism

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