PMID: 6159580Jul 11, 1980Paper

The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase

Nucleic Acids Research
M HoughtonA G Porter

Abstract

Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous with the mRNA sequence coding for amino acids 42-45 and used it as a primer to selectively transcribe an interferon cDNA template. The sequence of the newly synthesised DNA predicted the sequence of amino acids 48-109 in the interferon polypeptide. By repeating this process with one more primer, we have determined the complete amino acid sequence of mature human fibroblast interferon, a polypeptide of 166 amino acids.

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 11, 1976·Proceedings of the National Academy of Sciences of the United States of America·E Knight

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Citations

Jan 1, 1982·Archives of Virology·F H Sarkar, S L Gupta
Jan 1, 1985·Pharmacology & Therapeutics·J A Langer, S Pestka
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D Canaani, P Berg
Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·M D Edge, R Camble
Sep 1, 1982·The Journal of Experimental Medicine·A D SagarF H Ruddle
Feb 1, 1981·Journal of Interferon Research·E Knight, M W Hunkapiller
Jan 1, 1982·Journal of Interferon Research·E Dworkin-RastlP Swetly
Jan 1, 1983·Journal of Interferon Research·K G McCullaghM Houghton
Jan 1, 1985·Journal of Interferon Research·B LundE Lundgren
Jan 1, 1985·Journal of Interferon Research·L T May, P B Sehgal
Jun 25, 1982·Nucleic Acids Research·R DerynckD V Goeddel
Jan 24, 1981·Nucleic Acids Research·M HoughtonN H Carey
Mar 11, 1981·Nucleic Acids Research·R M LawnD V Goeddel
Oct 15, 1981·Biochemical and Biophysical Research Communications·W L TaylorJ E Dixon
Aug 20, 1981·Nature·M D EdgeA F Markham
Sep 30, 1982·Biochimica Et Biophysica Acta·P B Sehgal
Sep 30, 1982·Biochimica Et Biophysica Acta·M D Edge, A F Markham
Sep 30, 1982·Biochimica Et Biophysica Acta·M Rubinstein

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