PMID: 1367792Jan 1, 1991Paper

The complete amino acid sequence of the major Kunitz trypsin inhibitor from the seeds of Prosopsis juliflora

Phytochemistry
A N NegreirosMichael Richardson

Abstract

The major inhibitor of trypsin in seeds of Prosopsis juliflora was purified by precipitation with ammonium sulphate, ion-exchange column chromatography on DEAE- and CM-Sepharose and preparative reverse phase HPLC on a Vydac C-18 column. The protein inhibited trypsin in the stoichiometric ratio of 1:1, but had only weak activity against chymotrypsin and did not inhibit human salivary or porcine pancreatic alpha-amylases. SDS-PAGE indicated that the inhibitor has a Mr of ca 20,000, and IEF-PAGE showed that the pI is 8.8. The complete amino acid sequence was determined by automatic degradation, and by DABITC/PITC microsequence analysis of peptides obtained from enzyme digestions of the reduced and S-carboxymethylated protein with trypsin, chymotrypsin, elastase, the Glu-specific protease from S. aureus and the Lys-specific protease from Lysobacter enzymogenes. The inhibitor consisted of two polypeptide chains, of 137 residues (alpha chain) and 38 residues (beta chain) linked together by a single disulphide bond. The amino acid sequence of the protein exhibited homology with a number of Kunitz proteinase inhibitors from other legume seeds, the bifunctional subtilisin/alpha-amylase inhibitors from cereals and the taste-modifying pro...Continue Reading

References

Jan 1, 1975·Journal of Nutritional Science and Vitaminology·M KanamoriM Miyoshi
Mar 15, 1978·Biochemical and Biophysical Research Communications·D N Podell, G N Abraham
Jan 1, 1980·Journal of Agricultural and Food Chemistry·R Becker, O K Grosjean

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Citations

Feb 22, 2002·Biochemical and Biophysical Research Communications·Maria do Socorro M CavalcantiClaudio A M Sampaio
Dec 15, 2012·Plant Physiology and Biochemistry : PPB·Fabiola P S RufinoAdeliana S Oliveira
Oct 7, 2004·Archives of Biochemistry and Biophysics·Soghra Khatun HaqRizwan Hasan Khan
Feb 20, 2003·Archives of Biochemistry and Biophysics·M Fernanda TroncosoCarlota Wolfenstein-Todel
Jun 19, 2013·Plant Physiology and Biochemistry : PPB·Ana C B CruzElizeu A Santos
Jun 20, 2008·Phytochemistry·Navneet S ChaudharyAshwani K Sharma
Apr 22, 2015·Journal of Food Science and Technology·Saroj Kumar Prasad, Manoj Kumar Singh
Apr 7, 2010·Biochimie·Maria Luiza V OlivaMisako U Sampaio
Mar 7, 2014·The Science of the Total Environment·Clóvis Eduardo de Souza NascimentoJosé Cola Zanuncio
Mar 11, 2015·Pesticide Biochemistry and Physiology·Lays Cordeiro GuimarãesMaria Lígia Rodrigues Macedo
Aug 30, 2008·Phytochemistry·Alexander V KonarevPeter R Shewry
Apr 11, 2021·BMC Plant Biology·Franziska EberlSybille B Unsicker
Nov 11, 2008·Journal of Agricultural and Food Chemistry·Vanessa da Silveira RamosMaria Lígia Rodrigues Macedo
Jul 11, 2009·Journal of Agricultural and Food Chemistry·Kah Y EeSamson O Agboola
Sep 30, 2004·Journal of Agricultural and Food Chemistry·Francisco Delgado-VargasErwin de J López-Valenzuela
Oct 17, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Karina Margareti Alencar de BarrosMaria Lígia Rodrigues Macedo

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