The Complete Genome Sequence of the Murine Pathobiont Helicobacter typhlonius

Frontiers in Microbiology
Jeroen FrankSeyed Yahya Anvar

Abstract

Immuno-compromised mice infected with Helicobacter typhlonius are used to model microbially inducted inflammatory bowel disease (IBD). The specific mechanism through which H. typhlonius induces and promotes IBD is not fully understood. Access to the genome sequence is essential to examine emergent properties of this organism, such as its pathogenicity. To this end, we present the complete genome sequence of H. typhlonius MIT 97-6810, obtained through single-molecule real-time sequencing. The genome was assembled into a single circularized contig measuring 1.92 Mbp with an average GC content of 38.8%. In total 2,117 protein-encoding genes and 43 RNA genes were identified. Numerous pathogenic features were found, including a putative pathogenicity island (PAIs) containing components of type IV secretion system, virulence-associated proteins and cag PAI protein. We compared the genome of H. typhlonius to those of the murine pathobiont H. hepaticus and human pathobiont H. pylori. H. typhlonius resembles H. hepaticus most with 1,594 (75.3%) of its genes being orthologous to genes in H. hepaticus. Determination of the global methylation state revealed eight distinct recognition motifs for adenine and cytosine methylation. H. typhloni...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1997·International Journal of Systematic Bacteriology·J P Euzéby
Dec 24, 1998·Nucleic Acids Research·G Benson
Mar 31, 1999·Comparative Immunology, Microbiology and Infectious Diseases·L Zenner
Mar 24, 2000·Science·E W MyersJ C Venter
Oct 30, 2001·Journal of Clinical Microbiology·C L FranklinJ G Fox
Jun 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sebastian SuerbaumJames G Fox
Feb 5, 2004·Genome Biology·Stefan KurtzSteven L Salzberg
Apr 23, 2004·Infection and Immunity·Vincent B YoungDavid B Schauer
Oct 11, 2005·Nucleic Acids Research·Ross OverbeekVeronika Vonstein
Jan 5, 2006·Molecular Microbiology·Holly L Hamilton, Joseph P Dillard
Feb 21, 2006·Nature Reviews. Microbiology·Didier Wion, Josep Casadesús
Jul 25, 2006·Infection and Immunity·Jason S PrattVincent B Young
Jun 1, 2007·Nucleic Acids Research·Ibtissem GrissaChristine Pourcel
Feb 2, 2008·BMC Bioinformatics·Feng Gao, Chun-Ting Zhang
Feb 12, 2008·BMC Genomics·Ramy K AzizOlga Zagnitko
May 21, 2008·Cellular Microbiology·Zhongming GeJames G Fox
Nov 22, 2008·Science·John EidStephen Turner
Sep 17, 2009·Nature Reviews. Microbiology·Rémi FronzesGabriel Waksman
May 11, 2010·Nature Methods·Benjamin A FlusbergStephen W Turner
Mar 1, 2011·The FEBS Journal·Laurent Terradot, Gabriel Waksman
Jun 16, 2011·Nucleic Acids Research·You ZhouDavid S Wishart
Jul 4, 2012·Nature Biotechnology·Sergey Koren Adam M Phillippy
Jul 12, 2012·The Journal of Antimicrobial Chemotherapy·Ea ZankariMette Voldby Larsen
Sep 11, 2012·Applied and Environmental Microbiology· WasimuddinJaroslav Piálek
Oct 25, 2012·Nucleic Acids Research·Feng GaoChun-Ting Zhang
May 7, 2013·Nature Methods·Chen-Shan ChinJonas Korlach
Dec 3, 2013·Nucleic Acids Research·Ross OverbeekRick Stevens
Dec 5, 2013·Nucleic Acids Research·Juliane KrebesSebastian Suerbaum
Jan 9, 2014·Nucleic Acids Research·Diego GonzalezJustine Collier
Feb 28, 2014·Journal of Clinical Microbiology·Katrine Grimstrup JoensenFrank M Aarestrup
Aug 30, 2014·Helicobacter·Armelle MénardBram Flahou
Nov 8, 2014·Nucleic Acids Research·Richard J RobertsDana Macelis
Apr 30, 2015·Seminars in Speech and Language·Cynthia Core, Chiara Scarpelli
Sep 1, 2015·Carcinogenesis·Celia DingemanseEls C Robanus-Maandag

❮ Previous
Next ❯

Citations

Nov 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·John W BostickLiang Zhou

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJEB10402
LN907858

Methods Mentioned

BETA
PCR
Seq

Software Mentioned

RAST annotation service
T4SS
CRISPRFinder
NUCmer
Tandem Repeat Finder
R Core Team
Seed Viewer
VirulenceFinder
BLAST
PathogenFinder

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.