Dec 29, 2015

The complete mitochondrial genome of Lacerta bilineata and comparison with its closely related congener L. Viridis

Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
Sree Rohit KoloraM Schlegel

Abstract

We sequenced the mitochondrial genome of the Western green lizard (Lacerta bilineata) using Illumina technology and additional Sanger sequencing. The assembled 17 086 bp mitogenome had a GC content of 40.32% and consisted of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one control region (CR), with a gene order identical to the chordate consensus. In addition, we re-sequenced the mitogenome of the closely related Eastern green lizard L. viridis using the same techniques as for L. bilineata. The mitogenomes of L. bilineata and L. viridis showed a sequence identity of 94.4% and 99.9%, respectively, relative to the previously published L. viridis mitogenome. The phylogenetic reconstruction based on 17 Lacertinae mitogenomes using Anolis carolinensis as the outgroup supported L. bilineata and its sister species L. viridis as distinct lineages.

  • References12
  • Citations1

Mentioned in this Paper

Lepidopsetta bilineata
Lacerta viridis
Genes
DNA, Mitochondrial
MT-TA gene
Lochmaeus bilineata
Triplet Codon-amino Acid Adaptor Activity
Phylogenetic Analysis
Leiotettix viridis
Lebia viridis

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