The complete nucleotide sequence of an IncP-2 megaplasmid unveils a mosaic architecture comprising a putative novel blaVIM-2-harbouring transposon in Pseudomonas aeruginosa.

The Journal of Antimicrobial Chemotherapy
João BotelhoLuísa Peixe

Abstract

In Pseudomonas aeruginosa , bla VIM-2 has been mostly associated with a chromosomal location and rarely with a plasmid backbone. Until now, only three complete bla VIM-2 -carrying plasmid sequences have been described in this species. Here we explore the modular structure of pJB37, the first bla VIM-2 -carrying megaplasmid described in P. aeruginosa . The complete nucleotide sequence of plasmid pJB37 was determined with an Illumina HiSeq, with de novo assembly by SPAdes, annotation by RAST and searching for antimicrobial resistance genes and virulence factors. Conjugation assays were conducted. Megaplasmid pJB37 (464 804 bp long and GC content of 57.2%) comprised: an IncP-2 repA-oriV-parAB region; a conjugative transfer region ( traF , traG , virD2 and trbBCDEJLFGI genes); Tn 6356 , a new putative bla VIM-2 -carrying transposon; heavy metal (mercury and tellurite) resistance operons; and an arsenal of virulence genes. Plasmid pJB37 was transferable by conjugation to a spontaneous rifampicin-resistant mutant of P. aeruginosa PAO1. Here, a bla VIM-2 -harbouring In58 integron was associated with a new complex transposable structure, herein named Tn 6356 , suggesting that In58 was most likely acquired by insertion of this element. ...Continue Reading

References

Aug 1, 1983·Antimicrobial Agents and Chemotherapy·G A JacobyP Mammen
Mar 21, 2001·Antimicrobial Agents and Chemotherapy·S R PartridgeR M Hall
Jun 12, 2002·Journal of Bacteriology·Scott A BeatsonJohn S Mattick
Jan 31, 2007·Antimicrobial Agents and Chemotherapy·H W StokesRuth M Hall
Sep 10, 2008·Plasmid·Adam P RobertsDouglas E Berg
Feb 4, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C SantosS Mendo
May 21, 2010·The Journal of Antimicrobial Chemotherapy·Hayette TargantJean-Yves Madec
Jul 29, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A-P MagiorakosD L Monnet
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
Jul 4, 2012·Annual Review of Microbiology·Lori L Burrows
Sep 4, 2014·Antimicrobial Agents and Chemotherapy·Elisabet VilacobaDaniela Centrón
Apr 11, 2015·International Journal of Antimicrobial Agents·Anaïs PotronPatrice Nordmann
Jul 15, 2015·Infection & chemotherapy·Duck Jin HongKyungwon Lee
Aug 26, 2015·Antimicrobial Agents and Chemotherapy·Laurent DortetThierry Naas
Dec 19, 2015·The Journal of Antimicrobial Chemotherapy·Fengjun SunPeiyuan Xia

❮ Previous
Next ❯

Citations

May 8, 2019·Antimicrobial Agents and Chemotherapy·Anelise Stella BallabenAna Lucia Costa Darini
May 13, 2020·The Journal of Antimicrobial Chemotherapy·Xiaoyuan JiangDongsheng Zhou
Sep 21, 2018·Frontiers in Microbiology·Anneke van der ZeeJan Tommassen
Mar 5, 2020·The Journal of Antimicrobial Chemotherapy·Zhenpeng LiFang Bai
May 15, 2020·Clinical Microbiology Reviews·David M P De OliveiraMark J Walker
Mar 15, 2020·Nature Communications·Adrian CazaresCraig Winstanley
Aug 3, 2018·Clinical Microbiology Reviews·Sally R PartridgeSlade O Jensen
Dec 24, 2018·Mobile DNA·João BotelhoLuísa Peixe
Sep 8, 2019·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·João BotelhoLuísa Peixe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.