PMID: 4290550Apr 1, 1966Paper

The components of an alpha-glycerophosphate cycle and their relation to oxidative metabolism in the lens

The Biochemical Journal
M H Griffiths

Abstract

1. The concentration of ATP in a lens brei is maintained when the brei is incubated in oxygen with alpha-glycerophosphate. Lack of alpha-glycerophosphate or incubation in nitrogen causes the concentration to decrease. alpha-Glycerophosphate has some effect under anaerobic conditions but this is not sufficient to account for the maintenance in oxygen. 2. Manometric experiments show that alpha-glycerophosphate enhances the respiration of lens preparations. This respiration can be further increased by the addition of ADP and is abolished by cyanide and antimycin. The inference from these experiments is that a mitochondrial system able to oxidize alpha-glycerophosphate is present, i.e. the particulate half of the alpha-glycerophosphate cycle. 3. More than the calculated proportion of NADH is used when limiting amounts of dihydroxyacetone phosphate are added to lens tissue in spectrophotometric experiments. Dihydroxyacetone phosphate is therefore regenerated and an alpha-glycerophosphate cycle is operative. 4. A preparation of a particulate alpha-glycerophosphate dehydrogenase that takes up oxygen with methylene blue as electron acceptor is described. 5. Methods for obtaining mitochondria from lens are compared, and a useful extract...Continue Reading

Citations

Jan 1, 2010·The Libyan Journal of Medicine·Osama A Tashani, Mark I Johnson
Mar 1, 1981·Archives of Biochemistry and Biophysics·A K Chan, E A Thompson
Nov 1, 1971·Experimental Eye Research·P Trayhurn, R Van Heyningen

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