The Composition and Structure of Biofilms Developed by Propionibacterium acnes Isolated from Cardiac Pacemaker Devices

Frontiers in Microbiology
Ken-Ichi OkudaYoshimitsu Mizunoe

Abstract

The present study aimed to understand the biofilm formation mechanism of Propionibacterium acnes by analyzing the components and structure of the biofilms. P. acnes strains were isolated from the surface of explanted cardiac pacemaker devices that exhibited no clinical signs of infection. Culture tests using a simple stamp culture method (pressing pacemakers against the surface of agar plates) revealed frequent P. acnes colonization on the surface of cardiac pacemaker devices. P. acnes was isolated from 7/31 devices, and the isolates were categorized by multilocus sequence typing into five different sequence types (STs): ST4 (JK18.2), ST53 (JK17.1), ST69 (JK12.2 and JK13.1), ST124 (JK5.3), ST125 (JK6.2), and unknown ST (JK19.3). An in vitro biofilm formation assay using microtiter plates demonstrated that 5/7 isolates formed biofilms. Inhibitory effects of DNase I and proteinase K on biofilm formation varied among isolates. In contrast, dispersin B showed no inhibitory activity against all isolates. Three-dimensional live/dead imaging of P. acnes biofilms with different biochemical properties using confocal laser microscopy demonstrated different distributions and proportions of living and dead cells. Additionally, it was sugge...Continue Reading

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Citations

Jul 12, 2019·Frontiers in Microbiology·Andrei V GannesenMarc G J Feuilloley
Aug 28, 2020·Frontiers in Cellular and Infection Microbiology·Kaiyu ZhangYang Wang
Jan 26, 2021·Frontiers in Microbiology·Soshi SeikeHiroyasu Yamanaka
Jun 18, 2021·Frontiers in Microbiology·Holger BrüggemannRolf Lood
Aug 31, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yong-Guy KimJintae Lee

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Datasets Mentioned

BETA
ATCC
11828
KPA171202

Methods Mentioned

BETA
scanning electron microscopy
GAM
PCR
PCRs
Assay
Protein Assay
dot-blot

Software Mentioned

BLAST
Graph Pad Prism
ASEM
GraphPad

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