The composition of bovine peritubular dentin: matching TOF-SIMS, scanning electron microscopy and biochemical component distributions. New light on peritubular dentin function.

Cells, Tissues, Organs
Bat Ami Gotliv, Arthur Veis

Abstract

Peritubular dentin (PTD) is a hypermineralized phase within the dentinal tubules in some vertebrate teeth as an interface between the intertubular dentin (ITD) and the cell processes. Our aim has been to understand the composition, structure and role of PTD as a mineralized tissue. We have utilized the technique of time of flight secondary ion mass spectrometry (TOF-SIMS) to map the distribution of positive and negative inorganic ions as well as organic components in the fully mineralized, intact PTD structure in bovine tooth cross-sections, and correlated these with scanning electron microscopy (SEM) in standard and backscatter modes. In recent work, we developed a procedure to freeze fracture the teeth and separate PTD from the less dense ITD by the use of aqueous sodium phosphotungstate step density gradients, after degrading the ITD collagen with NaOCl. Here, PTD-containing fragments were characterized by SEM and TOF-SIMS surface structure analysis. The TOF-SIMS data show that the isolated PTD does not contain collagen, but its surface is rich in glutamic acid-containing protein(s). The TOF-SIMS spectra also indicated that the intact PTD fragments contain phospholipids, and chemical analyses showed phosphatidylserine, phosp...Continue Reading

References

Sep 1, 1967·Journal of Ultrastructure Research·E Bonucci
May 28, 1974·Calcified Tissue Research·N S PeressS W Sajdera
Jan 1, 1972·Calcified Tissue Research·H C SkinnerC Y Pak
May 15, 1993·Analytical Chemistry·D S MantusJ F Moulder
Oct 1, 2005·Analytical Chemistry·Sara G OstrowskiNicholas Winograd

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Citations

Sep 6, 2011·Micron : the International Research and Review Journal for Microscopy·Luiz Eduardo BertassoniMichael Vincent Swain
Jul 19, 2015·Journal of Endodontics·Maricela Garcés-OrtízJosé Reyes-Gasga
Nov 18, 2010·Journal of Structural Biology·Elia BeniashCharles S Sfeir
Apr 30, 2021·Journal of Conservative Dentistry : JCD·Morgane Marion KuntzeWilson Tadeu Felippe
Sep 30, 2018·Journal of Endodontics·Myrto PiperidouBettina R Basrani

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