The composition of chlorinated or oxidized phosphatidylcholine products changes with hypochlorite concentration: Application to abscess lipid analysis

Legal Medicine
Yumi HoshiokaHirotaro Iwase

Abstract

Hypochlorous acid, produced by myeloperoxidase upon neutrophil activation, can oxidize various compounds and exert antimicrobial activity in vivo. To elucidate the mechanisms underlying the reactions of the unsaturated phosphatidylcholines, which abound in cell membranes, with hypochlorous acid, we identified and examined phosphatidylcholine chlorination and oxidation products formed under various reaction conditions. We first investigated the products of unsaturated phosphatidylcholine and hypochlorous acid reaction with respect to hypochlorite concentration and reaction time. Next, we examined the lipids extracted postmortem from human abscesses. For all the analyses, we used liquid chromatography-quadrupole time-of-flight mass spectrometry. Various compounds, including phosphatidylcholine chlorohydrin and phosphatidylcholine hydroxide/epoxide, were detected. Oxidized phosphatidylcholines were mainly detectable upon reaction with low concentrations of sodium hypochlorite, whereas chlorinated phosphatidylcholines formed in the presence of higher concentrations. In human abscesses, oxidized phosphatidylcholines were detected in the cases with high procalcitonin concentration, whereas chlorinated phosphatidylcholines were undete...Continue Reading

References

Aug 1, 1992·Archives of Biochemistry and Biophysics·C C WinterbournF A Kuypers
Oct 24, 1995·Biochemical and Biophysical Research Communications·H IwaseK Takahashi
Aug 1, 1995·Free Radical Biology & Medicine·O M PanasenkoY A Vladimirov
Dec 8, 1994·Biochimica Et Biophysica Acta·O M PanasenkoV I Sergienko
Jun 1, 1994·Free Radical Biology & Medicine·M C VissersC C Winterbourn
Sep 1, 1995·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·J SchillerK Arnold
May 18, 2001·International Journal of Legal Medicine·M TsokosK Püschel
Mar 5, 2003·Free Radical Biology & Medicine·Oleg M PanasenkoJürgen Arnhold
Jul 19, 2003·Free Radical Research·Andreas JerlichCorinne M Spickett
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Mar 5, 2004·Chemistry and Physics of Lipids·Holger SpalteholzJürgen Arnhold
May 20, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Junko AdachiYasuhiro Ueno
Jul 2, 2005·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Naoki AikawaShinji Satomura
Jan 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sayuri MiyamotoPaolo Di Mascio
Dec 9, 2008·The International Journal of Biochemistry & Cell Biology·Mohammad Z AshrafEugene A Podrez
Mar 21, 2013·Current Atherosclerosis Reports·Maceler Aldrovandi, Valerie B O'Donnell
Nov 22, 2013·Journal of Mass Spectrometry : JMS·A ReisM R M Domingues
Mar 13, 2014·Archives of Biochemistry and Biophysics·Agnieszka RobaszkiewiczMirosław Soszyński
Feb 1, 1997·Redox Report : Communications in Free Radical Research·A J Kettle, C C Winterbourn
Mar 10, 2020·Journal of Thoracic Disease·Claudia GregorianoPhilipp Schuetz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.