The concentration dependence of the diffusion coefficient for bovine pancreatic trypsin inhibitor: a dynamic light scattering study of a small protein.

Biopolymers
W Gallagher, C K Woodward

Abstract

This paper reports the use of dynamic light scattering to investigate the concentration dependence of the diffusion coefficient for bovine pancreatic trypsin inhibitor (BPTI). BPTI is a small molecular weight protein (6511 Da) that has been the subject of numerous experimental studies. In addition to addressing questions that remain in the literature concerning the aggregation behavior of BPTI, we show that dynamic light scattering can be practically applied to proteins as small as BPTI, and that it can provide a useful means of parameterizing the solution behavior for proteins. We obtained values for the apparent diffusion coefficient of BPTI as a function of concentration over a range of pH values from 2.59 to 9.92 at an ionic strength of 0.3M, and over a range of ionic strength values from 0.1 to 0.5M at a pH of 7.0. The concentration dependence is linear for nearly all the conditions examined, even up to concentrations as high as 65 mg/mL. The average diffusion coefficient obtained at infinite dilution is 14.4 +/- 0.2 x 10(-7) cm2/s. This value agrees with that expected for a BPTI monomer hydrated with less than a monolayer of water. We used the theories of Felderhof, of Batchelor, and of Phillies, along with the DLVO theor...Continue Reading

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Citations

Dec 1, 1993·Protein Science : a Publication of the Protein Society·G I MakhatadzeP L Privalov
Sep 29, 2000·Journal of Chromatography. a·K Miyabe, G Guiochon
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Mar 17, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Bongkeun Kim, Xueyu Song
Apr 1, 1997·Proteins·M IkeguchiS Sugai

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