The concentration, extractability and characterization of collagen in human dental pulp

Archives of Oral Biology
J P van AmerongenG J Tonino

Abstract

Premolar and third molar dental pulps were studied. The amount of collagen in the dried pulps was 25.7 per cent in premolars and 31.9 per cent in third molars. These percentages are much higher than those reported for pulps in other species. Significant differences were further found in the collagen content and cell distribution (DNA) of the coronal, middle and apical parts of the pulp. Collagen content was the lowest in the coronal part, while the cell content was the lowest in the middle part. The extractability of collagen in a neutral salt solution or 0.5 M acetic acid was found to be extremely low (less than 1 per cent). Pretreatment of the pulp with hyaluronidase in order to remove proteoglycans had no effect on the solubility. It is concluded that human pulp collagen is highly cross-linked and cannot be considered as immature. Characterization of collagen was performed by methods in which limited pepsin digestion or CNBr cleavage was used. The digests were analysed by means of quantitative electrophoresis which revealed an amount of 42.6 per cent type III of the total collagen. Because of the large differences between dental pulps from man and experimental animals, extreme caution should be exercised in drawing conclusio...Continue Reading

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Citations

Aug 2, 2011·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Veronika PivodovaJitka Ulrichova
Nov 19, 2004·International Endodontic Journal·T MuramatsuM Shimono
Jan 1, 1990·Archives of Oral Biology·M TsuzakiG L Mechanic
Jan 1, 1984·Archives of Oral Biology·J P van AmerongenG J Tonino
Nov 1, 1992·Archives of Oral Biology·M Y KuoL J Hahn
Feb 19, 2008·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Alexandre M FernandesJosé B Alves
Oct 14, 2004·International Endodontic Journal·E F Martinez, V C Araújo
Oct 13, 2005·International Endodontic Journal·T MuramatsuM Shimono
Oct 15, 2014·International Endodontic Journal·M R ShinE-C Kim
Mar 15, 2005·Connective Tissue Research·Merja SulkalaLeo Tjäderhane
Jan 31, 2007·Brazilian Dental Journal·Ivete Jorge AbrahãoMárcia Martins Marques
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Jul 27, 2001·Journal of Endodontics·E F MartinezV Cavalcanti de Araújo
Apr 1, 1985·Journal of Dental Research·A Linde
Dec 11, 2020·Scientific Reports·Manal Matoug-ElwerfelliMonty Duggal

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