Abstract
The obligate intracellular parasite, Rickettsia prowazekii, is a slow-growing bacterium with a doubling time of about 10 h. In the present study, DNA and RNA were obtained from the rickettsiae by two independent methods, i.e. simultaneous isolation of DNA and RNA from the same sample by phenol:chloroform extraction and CsCI gradient centrifugation. In addition, ribosomal RNA was obtained by sedimentation of partially purified ribosomes from the rickettsiae. The results demonstrated that, after correction for the cell volumes, the concentrations of stable RNA and ribosomes in R. prowazekii, a slow-growing organism, were about 62 fg micron-3 and 17,000 per micron3, respectively, which were very similar (66 fg micron-3 and 21,000 per micron3) to those in Escherichia coli with a generation time of 40 min. However, on a per cell basis, R. prowazekii had 5.6 fg of RNA and 1500 ribosomes per cell, which was only about 8% of the amount of both stable RNA (71.2 fg) and ribosomes (24,000) per cell as was found in E. coli. These results indicated that R. prowazekii possesses a ribosome concentration greater than might have been predicted from its slow growth rate. This high concentration of ribosomes could be due to a large population of ...Continue Reading
References
Jan 1, 1977·Infection and Immunity·G A Dasch, E Weiss
Oct 1, 1976·Infection and Immunity·C L WissemanM R Jones
Oct 1, 1991·Antonie van Leeuwenhoek·A L Koch
Jan 17, 1986·Cell·R L GourseM Nomura
Sep 1, 1987·Infection and Immunity·H H Winkler
Jun 1, 1989·Infection and Immunity·J Turco, H H Winkler
Jan 1, 1989·Infection and Immunity·N A Buchmeier, F Heffron
Dec 1, 1988·Infection and Immunity·F E Austin, H H Winkler
Jul 1, 1974·Journal of Bacteriology·P P Dennis, H Bremer
Jul 1, 1970·Journal of Bacteriology·M J Pine
Feb 14, 1971·Journal of Molecular Biology·J Forchhammer, L Lindahl
Aug 28, 1971·Journal of Molecular Biology·R L CoffmanA L Koch
Jan 1, 1971·Advances in Microbial Physiology·A L Koch
Sep 1, 1993·Journal of Bacteriology·J Cai, H H Winkler
Jun 1, 1993·Journal of Bacteriology·K Z Abshire, F C Neidhardt
Dec 1, 1958·Journal of General Microbiology·M SCHAECHTERN O KJELDGAARD
May 1, 1964·Stain Technology·D F GIMENEZ
May 1, 1990·Journal of Food Protection·G L NortjéR J Grimbeek
Citations
May 1, 1996·Journal of Molecular Evolution·S G Andersson, P M Sharp
Jul 5, 2013·The ISME Journal·Steven J BlazewiczMary K Firestone
Feb 24, 2001·Cellular Microbiology·V A KempfI B Autenrieth
Sep 6, 2007·Genome Research·Amy K SchmidNitin S Baliga
Apr 7, 2005·Applied and Environmental Microbiology·Gerald D BaldridgeUlrike G Munderloh
Feb 6, 2007·Journal of Bacteriology·Les Dethlefsen, Thomas M Schmidt
Jan 8, 2005·BMC Bioinformatics·Les Dethlefsen, Thomas M Schmidt
Aug 6, 2011·Genome Biology·Charissa de BekkerHan A B Wösten
Jan 22, 2010·PLoS Genetics·Sara Vieira-Silva, Eduardo P C Rocha
Mar 24, 2000·Journal of Medical Entomology·J E WhitlockJ H Oliver
Jun 28, 2013·Environmental Microbiology·Bärbel U FoeselJörg Overmann
Aug 25, 2004·Journal of Microbiological Methods·Maaike C de VriesWillem M de Vos
Sep 24, 2015·The ISME Journal·Thomas S LankiewiczDavid L Kirchman
May 21, 2009·BMC Microbiology·Lalitha PeddireddiRoman R Ganta
Feb 8, 2011·PloS One·Andrew Woodard, David O Wood
Apr 13, 2007·Developmental and Comparative Immunology·Joshua T MattilaTimothy J Kurtti
May 13, 2017·The Journal of Eukaryotic Microbiology·Rao Fu, Jun Gong
Feb 27, 2018·FEMS Microbiology Ecology·Tina Šantl-TemkivKai Finster
Jan 16, 2018·Critical Reviews in Microbiology·Manlu Zhu, Xiongfeng Dai
Nov 12, 2016·Applied and Environmental Microbiology·Xiaowei ZhaoDaniela Nicastro
Jun 9, 2017·MSystems·Maria Agustina Domínguez-MartínJosé Manuel García-Fernández
Mar 17, 2017·ELife·Michael Lynch, Georgi K Marinov
Jun 15, 2019·Biology Direct·Marie GerlitzWilliam F Martin
Mar 16, 2019·Scientific Reports·Roby P BhattacharyyaDeborah T Hung
Jan 1, 1996·Microbiology·Leo EberlSøren Molin
Oct 31, 1998·Applied and Environmental Microbiology·F FegatellaR Cavicchioli
Jul 25, 2020·Science·Derek N MacklinMarkus W Covert
Mar 1, 1996·Journal of Bacteriology·H Pang, H H Winkler
Feb 1, 1995·Applied and Environmental Microbiology·S MollerS Molin
Jun 10, 1998·Journal of Clinical Microbiology·J A HigginsA F Azad
Dec 1, 1996·Applied and Environmental Microbiology·S MøllerS Molin
Oct 23, 1997·Journal of Bacteriology·E I ShawD O Wood
Oct 1, 1995·Journal of Bacteriology·L K PoulsenS Molin