Nov 8, 2018

The concerted action of E2-2 and HEB is critical for early lymphoid specification

BioRxiv : the Preprint Server for Biology
Thibault BouderliqueRobert Månsson

Abstract

The apparition of adaptive immunity in Gnathostomata correlates with the expansion of the E-protein family to encompass E2-2, HEB and E2A. Within the family, E2-2 and HEB are more closely evolutionarily related but their concerted action in hematopoiesis remains to be explored. Here we show that the combined disruption of E2-2 and HEB results in failure to express the early lymphoid program in CLPs and a near complete block in B-cell development. In the thymus, ETPs were reduced and T-cell development perturbed, resulting in reduced CD4 T- and increased γδ T-cell numbers. In contrast, HSCs, erythro-myeloid progenitors and innate immune cells were unaffected showing that E2-2 and HEB are dispensable for the ancestral hematopoietic lineages. Taken together, this E-protein dependence suggests that the appearance of the full Gnathostomata E-protein repertoire was critical to reinforce the gene regulatory circuits that drove the emergence and expansion of the lineages constituting humoral immunity.

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Mentioned in this Paper

Endopeptidase Clp Activity
T-Lymphocyte
Immune Effector Cell
Protein Family
Neoplasm of Uncertain or Unknown Behavior of Thymus
21-hydroxy-6,19-epithiopregn-4-ene-3,20-dione
Lymphoid Cells
TCF4
Disease of Thymus Gland
Humoral Immunity

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