PMID: 6164982Feb 25, 1981Paper

The conformation of chicken, rat and human U1A RNAs in solution

Nucleic Acids Research
C BranlantM Jacob

Abstract

Chicken, rat and human U1A RNAs in solution, were examined for secondary structure, using several methods including hydrolysis by various nucleases, hybridization to DNA oligomers and analysis of fragment interactions. The experimental results showed that the three U1A RNAs have the same structure, stable over a wide range of pH and ionic conditions. They allowed the selection of one out of several possible models constructed from the data of primary structure. This model is characterized by 4 hairpins and two single-stranded regions, the two hairpins from the 3' part of the molecule bearing very stable stems. In addition, the experimental results showed that in contrast to the 5' half of the molecule, the 3' half has a compact conformation probably stabilized by tertiary interactions. The 5' end of U1A RNA is accessible and free of base-pairing so that it might base-pair with regions of other RNA molecules, for instance, with the extremities of introns as has been recently proposed in a model of splicing.

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Citations

Feb 17, 2001·Biopolymers·David M. J. Lilley
Sep 1, 1992·Plant Molecular Biology·P VauxJ W Brown
Jun 1, 1989·Plant Molecular Biology·T KissF Solymosy
Jan 1, 1984·Journal of Molecular Evolution·P Hogeweg, D A Konings
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·V L van Santen, R A Spritz
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S L Deutscher, J D Keene
Dec 1, 1987·Journal of Biomolecular Structure & Dynamics·P RombyR Giegé
Feb 28, 2002·The EMBO Journal·Jeongsik YongGideon Dreyfuss
Sep 24, 1983·Nucleic Acids Research·W F MarzluffS S Wang
Jan 11, 1984·Nucleic Acids Research·K YamamotoH Yoshikura
Nov 25, 1987·Nucleic Acids Research·C EhresmannB Ehresmann
Jul 11, 1992·Nucleic Acids Research·V SégaultC Branlant
Mar 25, 1992·Nucleic Acids Research·C SturchlerA Krol
Sep 11, 1981·Nucleic Acids Research·H J Monstein, L Philipson
Nov 11, 1981·Nucleic Acids Research·R ReddyH Busch
Nov 25, 2003·Nucleic Acids Research·Marcel FreundHeiner Schaal
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·V LyamichevJ E Dahlberg
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·D G BreckenridgeM N Schnare
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Oct 5, 1987·Journal of Molecular Biology·J P Calvet, J A Myers
Jun 5, 1986·Journal of Molecular Biology·M N Lelay-TahaP Jeanteur
Sep 1, 1984·Bioscience Reports·R W Davies

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