The conformation of the mature dimeric human immunodeficiency virus type 1 RNA genome requires packaging of pol protein

Journal of Virology
M Shehu-XhilagaJ Mak

Abstract

The packaging of a mature dimeric RNA genome is an essential step in human immunodeficiency virus type 1 (HIV-1) replication. We have previously shown that overexpression of a protease (PR)-inactive HIV-1 Gag-Pro-Pol precursor protein generates noninfectious virions that contain mainly monomeric RNA (M. Shehu-Xhilaga, S. M. Crowe, and J. Mak, J. Virol. 75:1834-1841, 2001). To further define the contribution of HIV-1 Gag and Gag-Pro-Pol to RNA maturation, we analyzed virion RNA dimers derived from Gag particles in the absence of Gag-Pro-Pol. Compared to wild-type (WT) dimeric RNAs, these RNA dimers have altered mobility and low stability under electrophoresis conditions, suggesting that the HIV-1 Gag precursor protein alone is not sufficient to stabilize the dimeric virion RNA structure. The inclusion of an active viral PR, without reverse transcriptase (RT) and integrase (IN), rescued the stability of the virion RNA dimers in the Gag particles but did not restore the mobility of the RNAs, suggesting that RT and IN are also required for virion RNA dimer maturation. Thin-section electron microscopy showed that viral particles deficient in RT and IN contain empty cone-shaped cores. The abnormal core structure indicates a requireme...Continue Reading

References

Jan 1, 1992·Archives of Virology·J Y LeeD S Bowden
Jun 1, 1991·AIDS·J W Wills, R C Craven
Oct 1, 1988·The Journal of General Virology·D J HockleyA J Garrett
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E F TerwilligerW A Haseltine
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H G GöttlingerW A Haseltine
Dec 8, 1995·Journal of Molecular Biology·J L DarlixB P Roques
Jan 1, 1995·Biochimie·R MarquetB Ehresmann
Sep 1, 1993·Journal of Virology·W Fu, A Rein
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y X FengA Rein
Jan 1, 1996·Progress in Nucleic Acid Research and Molecular Biology·B Berkhout
Jan 1, 1996·Current Topics in Microbiology and Immunology·R C Craven, L J Parent
Dec 27, 1996·The Journal of Biological Chemistry·D MuriauxJ Paoletti
Sep 17, 1999·The Journal of Biological Chemistry·Y MorikawaK Sano
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·D MuriauxA Rein

❮ Previous
Next ❯

Citations

Nov 28, 2013·Expert Reviews in Molecular Medicine·Kellie Ann Jurado, Alan Engelman
Jan 28, 2006·AIDS Research and Human Retroviruses·Kelly R Young, Ted M Ross
Dec 25, 2008·Nucleic Acids Research·Cameron P KeatingJohnson Mak
Oct 11, 2007·RNA·Lucile SinckJean-Christophe Paillart
May 6, 2010·PLoS Computational Biology·Timothy E SchlubMiles P Davenport
Jun 27, 2007·FEBS Letters·Tat San LauDavid Chi Cheong Wan
Jan 5, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Edwin LeeansyahAnthony Jaworowski
May 3, 2018·Retrovirology·Paul D Bieniasz, Sebla B Kutluay
Aug 30, 2020·Viruses·Alex B Kleinpeter, Eric O Freed
Sep 13, 2020·Viruses·Jennifer L Elliott, Sebla B Kutluay
Dec 20, 2005·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·A G BukrinskayaM Stevenson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.