The conformational analysis of peptides using Fourier transform IR spectroscopy

Biopolymers
P I Haris, D Chapman

Abstract

Fourier transform infrared spectroscopy (FTIR) can be used for conformational analysis of peptides in a wide range of environments. Measurements can be performed in aqueous solution, organic solvents, detergent micelles as well as in phospholipid membranes. Information on the secondary structure of peptides can be derived from the analysis of the strong amide I band. Orientation of secondary structural elements within a lipid bilayer matrix can be determined by means of polarized attenuated total reflectance-FTIR spectroscopy. Hydrogen-deuterium exchange can be monitored by the analysis of the amide II band. This review gives some example of peptide systems studied by FTIR spectroscopy. Studies on alamethicin and alpha-aminoisobutyric acid containing peptides have shown that FTIR spectroscopy is a sensitive tool for identifying 3(10)-helical structures. Changes in the structure of the magainins upon interaction with charged lipids were detected using FTIR spectroscopy. Tachyplesin is an example of a beta-sheet containing membrane active peptide. Polarized ir spectroscopy reveals that the antiparallel beta-sheet structures of tachyplesin are oriented parallel to the membrane surface. Synthesis of peptides corresponding to functi...Continue Reading

References

Jun 1, 1992·Protein Engineering·S BoguszD D Busath
Sep 1, 1992·Trends in Biochemical Sciences·P I Haris, D Chapman
Oct 1, 1992·Physiological Reviews·O Pongs
Jun 1, 1990·Trends in Neurosciences·H R Guy, F Conti
Jan 1, 1991·Progress in Biophysics and Molecular Biology·M S Sansom
Jan 9, 1992·Biochimica Et Biophysica Acta·M Jackson, H H Mantsch
Aug 1, 1991·Current Opinion in Cell Biology·T M Perney, L K Kaczmarek
Nov 4, 1991·Biochimica Et Biophysica Acta·R BuchetA Martonosi
Feb 22, 1991·Science·H A HartmannA M Brown
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G R MarshallM T Leplawy
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H V WesterhoffM Zasloff
May 19, 1989·Biochimica Et Biophysica Acta·K MatsuzakiK Miyajima
Mar 1, 1986·Bioscience Reports·D C Lee, D Chapman
Jan 29, 1988·Biochimica Et Biophysica Acta·W K Surewicz, H H Mantsch
Jan 1, 1988·Annual Review of Biophysics and Biophysical Chemistry·M S Braiman, K J Rothschild
Aug 29, 1988·FEBS Letters·H C ChenC M Huang
Jan 1, 1986·Advances in Protein Chemistry·S Krimm, J Bandekar
Jan 1, 1986·Methods in Enzymology·H Susi, D M Byler

❮ Previous
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Citations

Dec 6, 2002·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Marek M Galka, Heinz-Bernhard Kraatz
May 16, 2002·Biopolymers·Jovencio HilarioTimothy A Keiderling
Jun 1, 1996·Bioorganic & Medicinal Chemistry·C L Nesloney, J W Kelly
Jan 1, 1996·Progress in Biophysics and Molecular Biology·P H Axelsen, M J Citra
Apr 27, 2000·Progress in Biophysics and Molecular Biology·L D BarronA F Bell
Jun 30, 1998·International Journal of Biological Macromolecules·D C BenakiS J Hamodrakas
May 16, 2002·Biochimica Et Biophysica Acta·Frédéric Heitz, Nicole Van Mau
Jul 6, 2001·Insect Biochemistry and Molecular Biology·V A IconomidouS J Hamodrakas
Jul 2, 2011·Biomacromolecules·Chongyi ChenZhibo Li
Jul 10, 2012·Biomacromolecules·Sabrina DehnSébastien Perrier
Sep 8, 2011·Journal of the American Chemical Society·Rami HouraniTing Xu
Jun 17, 2009·Journal of the American Chemical Society·Laura B SaglePaul S Cremer
Sep 22, 2009·Journal of the American Chemical Society·Toshiki SawadaHisakazu Mihara
Apr 5, 2012·Nano Letters·Jyoti KatochMasa Ishigami
Oct 16, 2012·ACS Nano·Sivan YuranMeital Reches
Aug 9, 2008·Physical Chemistry Chemical Physics : PCCP·Jun-Ho ChoiMinhaeng Cho
Aug 18, 2010·Physical Chemistry Chemical Physics : PCCP·Lin NiuChen Wang
May 1, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·D WangR W Roeske
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Roberto J BreaDirk M Guldi
Feb 16, 2002·Protein Science : a Publication of the Protein Society·Mireille DumoulinAndre Matagne
Feb 6, 1999·Annual Review of Physical Chemistry·R H CallenderW H Woodruff

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