PMID: 16637274Apr 28, 2006Paper

The conformational dynamic of the tetramer hemoglobin molecule as revealed by hydrogen exchange. I. Influence pH, temperature and ligand binding

Molekuliarnaia biologiia
L V AbaturovD A Faĭzullin

Abstract

The rate of the H-D exchange of the peptide NH atoms of the different forms of human Hb was studied at the range of pH 5-10 and temperature 10-63 degrees C by the IR spectroscopy. The pH-dependence of the H-D exchange rate is accordance with the EX2 mechanism. Two pH-dependent conformers of ligand forms of Hb existes at 10-30 degrees C with lower probability of local fluctuations of the alkaline conformer. The difference between two conformers vanishes at 40 degrees C with the appearance of the third conformer with higher probability of local fluctuations. The deoxyHb at 20 degrees C and pH range 6-9 has no pH-dependent conformers and the probability of local fluctuations is considerably reduced in comparison to the acid conformer of ligand Hb. Upon the destabilization of the ligand Hb structure by the pH decreasing to 5.0 at 20 degrees C or the temperature increasing up to 50-60 degrees C at pH 7.1 the global fluctuations of the native structure are intensified providing the H-D exchange of the slowest exchanging NH atoms. The nature of the local and global fluctuations and possible similarity between the two pH-dependent conformers of ligand Hb and its functional R and R2 states revealed by the X-ray analysis and NMR spectros...Continue Reading

References

Apr 5, 1979·Journal of Molecular Biology·J Baldwin, C Chothia
Aug 15, 1979·Journal of Molecular Biology·D A Case, M Karplus
May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Oct 1, 1980·Biophysical Journal·S W EnglanderJ R Rogero
Sep 1, 1994·Proteins·Y BaiS W Englander
Sep 1, 1993·Proteins·Y BaiS W Englander
Oct 28, 1997·Proceedings of the National Academy of Sciences of the United States of America·R HillerS W Englander
Apr 14, 1999·Nature Structural Biology·W A EatonA Mozzarelli
Oct 3, 1999·Nature Structural Biology·B M Huyghues-DespointesC N Pace
May 16, 2002·Proteins·Kazuaki Harata, Ryuta Kanai
Sep 11, 2002·Journal of Molecular Biology·Yue YuanChien Ho
Oct 9, 2002·Protein and Peptide Letters·Neeti Sinha, Sandra J Smith-Gill
Jan 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jonathan A LukinChien Ho
May 3, 2003·Current Opinion in Structural Biology·Rafael Brüschweiler
May 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Joan J EnglanderVirgil L Woods
Dec 18, 2003·Journal of the American Chemical Society·Michele VendruscoloMartin Karplus

❮ Previous
Next ❯

Citations

May 15, 2013·Journal of the American Society for Mass Spectrometry·Modupeola A Sowole, Lars Konermann
Mar 23, 2011·Journal of Molecular Biology·L MakowskiR F Fischetti
Nov 12, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jianbo LiuQinglin Sheng
Sep 5, 2013·Physical Chemistry Chemical Physics : PCCP·Yingdan QianChenxin Cai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.