PMID: 3773440Oct 1, 1986Paper

The congenic normal R/APfd and jaundiced R/APfd-j/j rat strains: a new animal model of hereditary non-haemolytic unconjugated hyperbilirubinaemia due to defective bilirubin conjugation

Laboratory Animals
R LeytenK P Heirwegh

Abstract

In this paper the production of the R/APfd-j/j strain which is congenic with the R/APfd strain is reported. The R/APfd-j/j completely lacks hepatic bilirubin UDP-glucuronyltransferase activity, as do our GUNNXR/Pfd-j/j rat strain and various other stocks of GUNN rats (j/j) described in the literature. Our recombinant inbred strain GUNNXR/Pfd-j/j was produced from non-inbred GUNN (j/j) rats. This GUNNXR/Pfd-j/j rat was used as a donor of the jaundice gene j, the R/APfd rat serving as the recipient. After eight backcross-intercross cycles (16 generations) the R/APfd-j/j strain was obtained which is congenic with the R/APfd strain. Congenicity was demonstrated by various techniques including transplantation of skin tissue, strain-specific tumour cells and hepatocytes, the mixed lymphocyte reaction, and comparison of biochemical markers. The potential of the novel inbred strain of jaundiced rat, R/APfd-j/j, and the corresponding control strain R/APfd for biochemical and clinical studies of bilirubin metabolism are briefly discussed.

Citations

Oct 14, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·P Kotal, J Fevery
May 15, 1988·Archives of Biochemistry and Biophysics·F Vanstapel, N Blanckaert
Nov 10, 1989·Journal of Chromatography·K P HeirweghN Blanckaert
Jan 1, 1989·Pharmacology & Therapeutics·B Burchell, M W Coughtrie
Jan 1, 1987·Molecular Aspects of Medicine·J FeveryN Blanckaert

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