The consequence of biologic graft processing on blood interface biocompatibility and mechanics

Cardiovascular Engineering and Technology
Aurore B Van De WallePeter S McFetridge

Abstract

Processing ex vivo derived tissues to reduce immunogenicity is an effective approach to create biologically complex materials for vascular reconstruction. Due to the sensitivity of small diameter vascular grafts to occlusive events, the effect of graft processing on critical parameters for graft patency, such as peripheral cell adhesion and wall mechanics, requires detailed analysis. Isolated human umbilical vein sections were used as model allogenic vascular scaffolds that were processed with either: 1. sodium dodecyl sulfate (SDS), 2. ethanol/acetone (EtAc), or 3. glutaraldehyde (Glu). Changes in material mechanics were assessed via uniaxial tensile testing. Peripheral cell adhesion to the opaque grafting material was evaluated using an innovative flow chamber that allows direct observation of the blood-graft interface under physiological shear conditions. All treatments modified the grafts tensile strain and stiffness properties, with physiological modulus values decreasing from Glu 240±12 kPa to SDS 210±6 kPa and EtAc 140±3 kPa, P<.001. Relative to glutaraldehyde treatments, neutrophil adhesion to the decellularized grafts increased, with no statistical difference observed between SDS or EtAc treatments. Early platelet adhe...Continue Reading

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Citations

Nov 3, 2017·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Victor E Rodríguez-RodríguezRodrigo Enrique Elizondo-Omaña
Dec 21, 2018·International Journal of Molecular Sciences·Andrea PorzionatoRaffaele De Caro
Feb 6, 2020·Journal of Tissue Engineering and Regenerative Medicine·Aurore B Van de WallePeter S McFetridge
Oct 30, 2021·Macromolecular Bioscience·Mahyar SametiChris A Bashur

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