The conserved B3 domain of VIVIPAROUS1 has a cooperative DNA binding activity

The Plant Cell
M SuzukiDonald R McCarty

Abstract

The biochemical activities that underlie the genetically defined activator and repressor functions of the VIVIPAROUS1 (VP1) protein have resisted in vitro analysis. Here, we show that a glutathione S-transferase (GST) fusion protein, including only the highly conserved B3 domain of VP1, has a highly cooperative, sequence-specific DNA binding activity. GST fusion proteins that include larger regions of the VP1 protein have very low activity, indicating that removal of the flanking protein sequences is necessary to elicit DNA binding in vitro. DNA competition and DNase I footprinting analyses show that B3 binds specifically to the Sph element involved in VP1 activation of the C1 gene, whereas binding to the G-box-type VP1-responsive element is of low affinity and is nonspecific. Footprint analysis of the C1 promoter revealed that sequences flanking the core TCCATGCAT motif of Sph also contribute to the recognition of the Sph element in its native context. The salient features of the in vitro GST-B3 DNA interaction are in good agreement with the protein and DNA sequence requirements defined by the functional analyses of VP1 and VP1-responsive elements in maize cells.

References

Oct 1, 1992·The Plant Cell·J GiraudatH M Goodman
Oct 1, 1989·The Plant Cell·W R MarcotteR S Quatrano
Mar 1, 1994·Plant Molecular Biology·T HattoriS T Hamasuna

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Citations

Jan 1, 2002·The Arabidopsis Book·Toru FujiwaraSatoshi Naito
Jan 1, 2002·The Arabidopsis Book·Ruth R Finkelstein, Christopher D Rock
Feb 5, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Fred Y Peng, Randall J Weselake
Mar 19, 2011·Journal of Plant Research·Daisuke TakezawaYoichi Sakata
Mar 19, 2011·Journal of Plant Research·Yasunari FujitaKazuko Yamaguchi-Shinozaki
Dec 18, 2004·Plant Molecular Biology·Raúl CarrancoTimothy C Hall
Nov 27, 2007·Plant Molecular Biology·Danny W-K Ng, Timothy C Hall
Jan 1, 2010·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·S AnandhanK R Koundal
Jan 13, 2011·Journal of Applied Genetics·A Gruszczyńska, M Rakoczy-Trojanowska
May 16, 2000·Plant Science : an International Journal of Experimental Plant Biology·A J CroweM M Moloney
Mar 6, 1999·Current Opinion in Plant Biology·E Grill, A Himmelbach
Feb 27, 1999·Current Opinion in Plant Biology·U Wobus, H Weber
Sep 4, 1999·The Plant Journal : for Cell and Molecular Biology·T UlmasovT J Guilfoyle
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·T HoboT Hattori
Aug 8, 2001·The Plant Journal : for Cell and Molecular Biology·S NakamuraR R Finkelstein
Dec 12, 2001·The Plant Journal : for Cell and Molecular Biology·M SuzukiD R McCarty
Nov 2, 2002·The Plant Journal : for Cell and Molecular Biology·Luis Lopez-MolinaNam-Hai Chua
Mar 6, 2009·Journal of Experimental Botany·Andrés P GageteM Isabel Rodrigo
Jun 26, 2012·Nucleic Acids Research·Gudrun MönkeLothar Altschmied
Feb 14, 2002·Plant Physiology·José M Franco-ZorrillaJosé M Martínez-Zapater
Jan 17, 2003·Plant Physiology·Inès M Brocard-GiffordRuth R Finkelstein

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