PMID: 8609202Nov 1, 1995Paper

The conserved, hydrophilic and arginine-rich N-terminal domain of cucumovirus coat proteins contributes to their anomalous electrophoretic mobilities in sodium dodecylsulfate-polyacrylamide gels

Journal of Virological Methods
C C Hu, S A Ghabrial

Abstract

Although the Mr values of the coat proteins (CPs) of several cucumoviruses have been calculated from their deduced amino acid sequences to be approximately 24,000, the experimentally determined M(r) values using the Laemmli SDS-PAGE system were 30,000-31,000. Examination of the amino acid composition revealed that these CPs are neither highly acidic nor highly basic. Post-translational glycosylation or phosphorylation were also ruled out as contributing factors to the observed anomalous electrophoretic mobility because the products of in vitro translation of cucumovirus RNA 4 and in vivo bacterial expression of the cloned CP gene co-migrated with authentic cucumovirus CPs. Comparison of the hydropathy profiles of the CPs revealed the presence in each of a strikingly similar, highly hydrophilic N-terminal domain of 30-32 amino acid residues that contains a cluster of basic amino acids, mainly arginine. Selective chemical cleavage at tryptophan residues in the CPs of cucumoviruses, known to contain single tryptophan residues, yielded two peptides; an N-terminal peptide that contained the conserved hydrophilic domain and a C-terminal peptide. SDS-PAGE analysis showed that the N-terminal, but not the C-terminal, peptide exhibited t...Continue Reading

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Citations

Jan 13, 2004·Biochemical and Biophysical Research Communications·Viviane S AlvesBeatriz A Castilho
May 27, 2005·Biochemical and Biophysical Research Communications·Gang-hua LangKiyotaka Miyashita
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·P XuH G Zot
Jun 23, 2001·Protein Science : a Publication of the Protein Society·L M IakouchevaE J Ackerman
Dec 24, 2010·Journal of Virology·Desislava A RaytchevaJonathan A King
Jun 23, 2005·BMC Plant Biology·Julia KehrPatrick Giavalisco
Feb 22, 2002·Biochemical and Biophysical Research Communications·Kousei NoguchiToru Higashinakagawa
Dec 7, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Fabien CôtéDenis Leclerc
Jun 6, 2009·The FEBS Journal·David S LibichSteven M Pascal
Nov 16, 2005·Proteins·Véronique Receveur-BréchotSonia Longhi
Jan 10, 2006·Proteomics·Patrick GiavaliscoJulia Kehr
Feb 9, 2007·American Journal of Physiology. Cell Physiology·Ying GuoPhilip C Trackman

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