Aug 15, 2014

The conserved PFT1 tandem repeat is crucial for proper flowering in Arabidopsis thaliana

Genetics
Pauline RivalChristine Queitsch

Abstract

It is widely appreciated that short tandem repeat (STR) variation underlies substantial phenotypic variation in organisms. Some propose that the high mutation rates of STRs in functional genomic regions facilitate evolutionary adaptation. Despite their high mutation rate, some STRs show little to no variation in populations. One such STR occurs in the Arabidopsis thaliana gene PFT1 (MED25), where it encodes an interrupted polyglutamine tract. Although the PFT1 STR is large (∼270 bp), and thus expected to be extremely variable, it shows only minuscule variation across A. thaliana strains. We hypothesized that the PFT1 STR is under selective constraint, due to previously undescribed roles in PFT1 function. We investigated this hypothesis using plants expressing transgenic PFT1 constructs with either an endogenous STR or synthetic STRs of varying length. Transgenic plants carrying the endogenous PFT1 STR generally performed best in complementing a pft1 null mutant across adult PFT1-dependent traits. In stark contrast, transgenic plants carrying a PFT1 transgene lacking the STR phenocopied a pft1 loss-of-function mutant for flowering time phenotypes and were generally hypomorphic for other traits, establishing the functional import...Continue Reading

  • References37
  • Citations4

References

  • References37
  • Citations4

Citations

Mentioned in this Paper

Short Tandem Repeat
Conserved Sequence
Genes, Plant
Plant Carpals
Transcription Repressor/Corepressor
Tandem Repeat Sequences
Genome
Molecular_function
Candidate Gene Identification
Gene Expression Regulation, Plant

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