The Construction and Analysis of lncRNA-miRNA-mRNA Competing Endogenous RNA Network of Schwann Cells in Diabetic Peripheral Neuropathy

Frontiers in Bioengineering and Biotechnology
Cheng WangYanhua Chen

Abstract

Diabetes mellitus is a worldwide disease with high incidence. Diabetic peripheral neuropathy (DPN) is one of the most common but often ignored complications of diabetes mellitus that cause numbness and pain, even paralysis. Recent studies demonstrate that Schwann cells (SCs) in the peripheral nervous system play an essential role in the pathogenesis of DPN. Furthermore, various transcriptome analyses constructed by RNA-seq or microarray have provided a comprehensive understanding of molecular mechanisms and regulatory interaction networks involved in many diseases. However, the detailed mechanisms and competing endogenous RNA (ceRNA) network of SCs in DPN remain largely unknown. Whole-transcriptome sequencing technology was applied to systematically analyze the differentially expressed mRNAs, lncRNAs and miRNAs in SCs from DPN rats and control rats. Gene ontology (GO) and KEGG pathway enrichment analyses were used to investigate the potential functions of the differentially expressed genes. Following this, lncRNA-mRNA co-expression network and ceRNA regulatory network were constructed by bioinformatics analysis methods. The results showed that 2925 mRNAs, 164 lncRNAs and 49 miRNAs were significantly differently expressed in SCs...Continue Reading

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Citations

Jun 18, 2021·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Chengqi YanZhenbing Chen

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Datasets Mentioned

BETA
AABR07030791.1
AABR07054488.1
AABR07036331.2
AABR07061328.1
AABR07063724.1
GSE142785

Methods Mentioned

BETA
RNA-seq
blood draws
transmission electron microscopy
PCR
Assay

Software Mentioned

featureCounts
HISAT2
Cytoscape
Stringtie
TargetScan
DESeq2
Image J
Bowtie
fastp
miRanda

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