PMID: 6110205Nov 25, 1980Paper

The construction of a recombinant cDNA library representative of the poly(A)+ mRNA population from normal human lymphocytes

Nucleic Acids Research
D WoodsR Williamson

Abstract

A recombinant library has been constructed using the plasmid pAT153 and double stranded cDNA prepared from normal human lymphocyte poly(A)+ RNA. Transformation conditions were optimized to yield approximately 200,000 recombinants per microgram of double stranded cDNA. Statistical analysis as well as sequence complexity analysis of the inserted sequences indicates that the cDNA library is representative of > 99% of the poly(A)+ RNA present in the normal human lymphocyte.

References

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Citations

Jan 1, 1985·Toxicon : Official Journal of the International Society on Toxinology·M L VandenplasC D Boyd
Nov 22, 1984·Biochimica Et Biophysica Acta·D S LatchmanM J Evans
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D E WoodsH R Colten
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J N Davidson, L A Niswander
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D BoydJ Drysdale
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M I LomaxL I Grossman
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B AlarconH Spits
Nov 25, 1983·Nucleic Acids Research·L M KunkelS A Latt
Oct 10, 1981·Nucleic Acids Research·S E HumphriesR Williamson
Mar 11, 1982·Nucleic Acids Research·J E Arrand, A M Murray
Oct 31, 1983·Biochemical and Biophysical Research Communications·J RogersS E Humphries
Jul 30, 1982·Biochimica Et Biophysica Acta·J E ArrandR Williamson
Dec 1, 1981·European Journal of Biochemistry·I J JacksonR Williamson

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