PMID: 3320049Dec 1, 1987Paper

The contact site A glycoprotein mediates cell-cell adhesion by homophilic binding in Dictyostelium discoideum

The Journal of Cell Biology
C H SiuA H Choi

Abstract

Dictyostelium discoideum expresses a developmentally regulated cell surface glycoprotein of Mr 80,000 (gp80), which has been implicated in the formation of the EDTA-resistant contact sites A at the cell aggregation stage. To determine whether gp80 participates directly in cell binding and, if so, its mode of action, we conjugated purified gp80 to Covaspheres (Covalent Technology Corp., Ann Arbor, MI) and investigated their ability to bind to cells. The binding of gp80-Covaspheres was dependent on the developmental stage of the cells, with maximal interaction at the late aggregation stage. Scanning electron microscopic studies revealed the clustering of gp80-Covaspheres at the polar ends of these cells, similar to the pattern of gp80 distribution on the cell surface as reported earlier (Choi, A. H. C., and Siu, C.-H., 1987, J. Cell Biol., 104:1375-1387). Precoating cells with an adhesion-blocking anti-gp80 monoclonal antibody inhibited the binding of gp80-Covaspheres, suggesting that Covasphere-associated gp80 might undergo homophilic interaction with gp80 on the cell surface. Quantitative binding of 125I-labeled gp80 to intact cells gave an estimate of 1.5 X 10(5) binding sites per cell at the aggregation stage. Binding of solu...Continue Reading

References

May 1, 1975·Developmental Biology·P C Letourneau
Jun 1, 1986·The Journal of Cell Biology·A N van den PolU Rutishauser
Nov 1, 1986·The Journal of Cell Biology·A L Frelinger, U Rutishauser
May 1, 1985·Developmental Biology·W R Springer, S H Barondes
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H C TsuiW L Klein
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S D RosenS H Barondes
May 1, 1970·The Journal of Cell Biology·S M Cocucci, M Sussman
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B A CunninghamG M Edelman
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C H SiuT Y Lam
Feb 4, 1983·Science·G M Edelman
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·U RutishauserG M Edelman
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·B A MurrayW F Loomis

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Citations

Aug 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C H Siu
Jan 1, 1990·Developmental Genetics·C H Siu, R K Kamboj
Jul 1, 1992·Journal of Cellular Physiology·C H SiuI B Fritz
Dec 18, 1995·Experientia·S Bozzaro, E Ponte
Aug 1, 1995·Quarterly Reviews of Biophysics·R Grisshammer, C G Tate
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·E PonteS Bozzaro
Jul 1, 1993·Differentiation; Research in Biological Diversity·D R Fontana
Apr 15, 2015·Developmental Biology·William F Loomis
Mar 30, 2001·The Journal of Biological Chemistry·T J HarrisC H Siu
Dec 14, 1999·Biochimica Et Biophysica Acta·C ChanchaoC L Rutherford

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