The contribution of Chlamydia-specific CD8⁺ T cells to upper genital tract pathology

Immunology and Cell Biology
Kelly R VlcekAshlesh K Murthy

Abstract

Genital chlamydial infections lead to severe upper reproductive tract pathology in a subset of untreated women. We demonstrated previously that tumor necrosis factor (TNF)-α-producing CD8(+) T cells contribute significantly to chlamydial upper genital tract pathology in female mice. In addition, we observed that minimal chlamydial oviduct pathology develops in OT-1 transgenic (OT-1) mice, wherein the CD8(+) T-cell repertoire is restricted to recognition of the ovalbumin peptide Ova(257-264), suggesting that non-Chlamydia-specific CD8(+) T cells may not be responsible for chlamydial pathogenesis. In the current study, we evaluated whether antigen-specific CD8(+) T cells mediate chlamydial pathology. Groups of wild-type (WT) C57BL/6J, OT-1 mice, and OT-1 mice replete with WT CD8(+) T cells (1 × 10(6) cells per mouse intravenously) were infected intravaginally with C. muridarum (5 × 10(4) IFU/mouse). Serum total anti-Chlamydia antibody and total splenic anti-Chlamydia interferon (IFN)-γ and TNF-α responses were comparable among the three groups of animals. However, Chlamydia-specific IFN-γ and TNF-α production from purified splenic CD8(+) T cells of OT-1 mice was minimal, whereas responses in OT-1 mice replete with WT CD8(+) T cel...Continue Reading

References

Apr 1, 1983·British Medical Bulletin·L Weström, P A Mårdh
Jan 1, 1994·Methods in Enzymology·R G Rank
Jun 1, 1996·The Journal of Infectious Diseases·J KimaniR C Brunham
May 16, 2002·Infection and Immunity·Richard P Morrison, Harlan D Caldwell
Jul 14, 2004·Nature Reviews. Microbiology·Robert BellandGerald I Byrne
Jul 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·David E NelsonHarlan D Caldwell
Aug 7, 2009·The Journal of Infectious Diseases·Joseph U IgietsemeCarolyn M Black
Feb 1, 2013·Infectious Diseases in Obstetrics and Gynecology·Janina JiangKathleen A Kelly
Apr 9, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Norma Olivares-ZavaletaHarlan D Caldwell
Feb 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catarina V NogueiraMichael N Starnbach

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Citations

Mar 5, 2016·Expert Review of Vaccines·Hong YuRobert C Brunham
Nov 12, 2016·Human Vaccines & Immunotherapeutics·Steven LiangJames Mahony
Sep 8, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Juan Pablo Mackern-ObertiVirginia Elena Rivero
Aug 11, 2017·PloS One·Yuyang ZhangGuangming Zhong
Mar 13, 2019·Infection and Immunity·Tianyuan ZhangGuangming Zhong
Jan 4, 2020·The Journal of Infectious Diseases·Raymond M JohnsonGang Peng
Jan 29, 2020·Infection and Immunity·Qi TianGuangming Zhong
Mar 25, 2020·Infection and Immunity·Zhi HuoGuangming Zhong
Aug 5, 2020·Infection and Immunity·Lingxiang XieGuangming Zhong
Oct 23, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Clare E GyorkeUma M Nagarajan
Feb 5, 2021·Pathogens and Disease·Jacob Dockterman, Jörn Coers

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Methods Mentioned

BETA
flow cytometry
ELISA

Software Mentioned

Sigma Stat

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