The contribution of polystyrene nanospheres towards the crystallization of proteins.

PloS One
Johanna M KallioJuha Rouvinen

Abstract

Protein crystallization is a slow process of trial and error and limits the amount of solved protein structures. Search of a universal heterogeneous nucleant is an effort to facilitate crystallizability of proteins. The effect of polystyrene nanospheres on protein crystallization were tested with three commercial proteins: lysozyme, xylanase, xylose isomerase, and with five research target proteins: hydrophobins HFBI and HFBII, laccase, sarcosine dimethylglycine N-methyltransferase (SDMT), and anti-testosterone Fab fragment 5F2. The use of nanospheres both in screening and as an additive for known crystallization conditions was studied. In screening, the addition of an aqueous solution of nanosphere to the crystallization drop had a significant positive effect on crystallization success in comparison to the control screen. As an additive in hydrophobin crystallization, the nanospheres altered the crystal packing, most likely due to the amphiphilic nature of hydrophobins. In the case of laccase, nanospheres could be used as an alternative for streak-seeding, which insofar had remained the only technique to produce high-diffracting crystals. With methyltransferase SDMT the nanospheres, used also as an additive, produced fewer, la...Continue Reading

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Citations

Jun 13, 2014·Nature Protocols·Sahir KhurshidNaomi E Chayen
Aug 5, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Juha Pekka KallioJuha Rouvinen
Sep 9, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Javier García-NafríaKeith S Wilson
Aug 3, 2011·Chemical Communications : Chem Comm·Johanna M Kallio, Juha Rouvinen
Jul 27, 2012·Nanoscale·Benjamin S GullyCharles S Bond
Mar 2, 2011·Journal of Molecular Recognition : JMR·Merja H NiemiMatti Höyhtyä
Mar 23, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Anindita Sengupta Ghatak, Animangsu Ghatak

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Methods Mentioned

BETA
X-ray
phage display
atomic force microscopy

Software Mentioned

Crystal Screen HT
Crystal
SHELXL

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