The Contribution of Sulfated Glycosaminoglycans to the Inflation Response of the Human Optic Nerve Head

Investigative Ophthalmology & Visual Science
Dan MidgettThao D Nguyen

Abstract

In this study, we measured the effect of the removal of sulfated glycosaminoglycans (sGAGs) on the pressure-induced strains of the human lamina cribrosa (LC). We applied an ex vivo inflation method to measure the three-dimensional (3D) deformation response of six human LCs to pressure, before and after the degradation of chondroitin and dermatan sulfates. The experiment used a laser-scanning microscope (LSM) to acquire the second harmonic generation (SHG) signal of the collagen structure in the LC. Digital volume correlation (DVC) was used to calculate the deformation in the LC after a change in pressure from 5 to 45 mm Hg. The average strains between 5 and 45 mm Hg in the LC decreased significantly after sGAG degradation (P ≤ 0.03), with the greatest change occurring in regions of previously high strain (P ≤ 0.003) and the peripheral regions of the LC (P ≤ 0.02). The stiffening effect was greater in the LC of middle-aged (42-49 years) donors compared with those of older (64-88 years) donors (P < 0.0001). The LC experienced less strain at the same pressures after most sGAGs were removed. These results suggest that the natural decrease in sGAGs within the LC with age may contribute to the stiffer inflation response of older LC t...Continue Reading

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Citations

Apr 29, 2020·Investigative Ophthalmology & Visual Science·Dan MidgettThao D Nguyen
Jun 4, 2019·Investigative Ophthalmology & Visual Science·Yik Tung Tracy LingThao D Nguyen
Nov 10, 2020·Investigative Ophthalmology & Visual Science·Andrew P VoorheesIan A Sigal
Aug 15, 2019·Progress in Retinal and Eye Research·Craig BooteMichael J A Girard
Jul 17, 2021·Orphanet Journal of Rare Diseases·Weijing KongYan Meng

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