The contribution of Tannerella forsythia dipeptidyl aminopeptidase IV in the breakdown of collagen

Molecular Oral Microbiology
Susan Yost, Ana E Duran-Pinedo

Abstract

In this study, we characterized a serine protease from Tannerella forsythia that degrades gelatin, type I, and III collagen. Tannerella forsythia is associated with periodontitis progression and severity. The primary goal of this research was to understand the mechanisms by which T. forsythia contributes to periodontitis progression. One of our previous metatranscriptomic analysis revealed that during periodontitis progression T. forsythia highly expressed the bfor_1659 ORF. The N-terminal end is homologous to dipeptidyl aminopeptidase IV (DPP IV). DPP IV is a serine protease that cleaves X-Pro or X-Ala dipeptide from the N-terminal end of proteins. Collagen type I is rich in X-Pro and X-Ala sequences, and it is the primary constituent of the periodontium. This work assessed the collagenolytic and gelatinolytic properties of BFOR_1659. To that end, the complete BFOR_1659 and its domains were purified as His-tagged recombinant proteins, and their collagenolytic activity was tested on collagen-like substrates, collagen type I and III combined, and on the extracellular matrix (ECM) formed on human gingival fibroblasts culture HGF-1. BFOR_1659 was only found in T. forsythia supernatants, highlighting its potential role on the patho...Continue Reading

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Citations

Jul 25, 2019·Molecular Oral Microbiology·Yeon-Kyeong KoBong-Kyu Choi
Nov 24, 2020·Periodontology 2000·Ana E Duran-Pinedo
Jul 17, 2021·Critical Reviews in Microbiology·Simón ÁlvarezSebastian Aguayo
Nov 4, 2021·Journal of Oral Biosciences·Anthony LuongDalia Seleem

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