The contribution of the Tie2+ lineage to primitive and definitive hematopoietic cells.

Genesis : the Journal of Genetics and Development
Yuefeng TangLucy Liaw

Abstract

The regulatory elements of the Tie2/Tek promoter are commonly used in mouse models to direct transgene expression to endothelial cells. Tunica intima endothelial kinase 2 (Tie2) is also expressed in hematopoietic cells, although this has not been fully characterized. We determine the lineages of adult hematopoietic cells derived from Tie2-expressing populations using Tie2-Cre;Rosa26R-EYFP mice. In Tie2-Cre;Rosa26R-EYFP mice, analysis of bone marrow cells showed Cre-mediated recombination in 85% of the population. In adult bone marrow and spleen, we analyzed subclasses of early hematopoietic progenitors, T cells, monocytes, granulocytes, and B cells. We found that ∼ 84% of each lineage was EYFP(+), and nearly all cells that come from Tie2-expressing lineages are CD45(+), confirming widespread contribution to definitive hematopoietic cells. In addition, more than 82% of blood cells within the embryonic yolk sac were of Tie2(+) origin. Our findings of high levels of Tie2-Cre recombination in the hematopoietic lineage have implications for the use of the Tie2-Cre mouse as a lineage-restricted driver strain.

References

Feb 13, 2001·Developmental Biology·Y Y KisanukiM Yanagisawa
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J PalisM C Yoder
Aug 31, 2002·Genesis : the Journal of Genetics and Development·Anne FordeBernd Arnold
Oct 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mira C Puri, Alan Bernstein
Sep 6, 2005·Experimental Hematology·Kathleen E McGrath, James Palis
Mar 23, 2007·Nature·Igor M SamokhvalovShin-ichi Nishikawa
Dec 2, 2008·Cell Stem Cell·Ann C ZoveinM Luisa Iruela-Arispe

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Citations

Jul 26, 2012·Science Signaling·Chang-Lung LeeDavid G Kirsch
May 26, 2012·Circulation Research·Thomas Doetschman, Mohamad Azhar
Jan 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·S Leah EtheridgeIan S Hitchcock
Mar 4, 2014·Cell Death and Differentiation·I CorteganoJ L de la Pompa
Aug 26, 2011·Cell and Tissue Research·Laurens A van Meeteren, Peter ten Dijke
Aug 1, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Eileen McNeillKeith M Channon
Feb 14, 2016·Developmental Biology·Nijiro NohataJ Silvio Gutkind
Dec 1, 2015·Nature Communications·Smail MessaoudiMona Nemer
Mar 13, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hwanhee OhJang-Soo Chun
Feb 3, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jason E Fish, Joshua D Wythe
Oct 13, 2012·Mechanisms of Development·Oliver K AppelbeJennifer V Schmidt
Apr 11, 2014·Blood·Lesley A EverettDavid Ginsburg
May 8, 2016·Developmental Biology·Jerfiz D ConstanzoPier Paolo Scaglioni
Oct 26, 2016·Differentiation; Research in Biological Diversity·Yu Bo Yang SunJinhua Li
Jul 23, 2016·Nature Communications·Onur KanisicakJeffery D Molkentin
Jan 10, 2017·Developmental Biology·Travis Brooke-BisschopDavid Lohnes
Oct 19, 2017·International Journal of Molecular Sciences·Evangelia PardaliPeter Ten Dijke
Jan 23, 2018·Circulation Journal : Official Journal of the Japanese Circulation Society·Yukio HiroiJames K Liao
Mar 24, 2018·Genesis : the Journal of Genetics and Development·Hyea Jin GilGuillermo Oliver
May 24, 2018·Journal of Leukocyte Biology·Maryna SamusDietmar Vestweber
Jun 12, 2018·Physiological Reports·Ramesh ChennupatiS Eleonore Koehler
Jan 9, 2016·Journal of Cell Science·Sanna Vattulainen-CollanusTero-Pekka Alastalo
Apr 5, 2013·Cellular Reprogramming·Aaron SchindelerDavid G Little
Jun 23, 2018·Circulation Research·Mengcheng ShenZamaneh Kassiri
Aug 31, 2018·European Journal of Immunology·Christoph Konradt, Christopher A Hunter
Sep 25, 2018·Journal of Cellular Physiology·Vahid SiavashiAli Mohammad Sharifi
May 6, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Yao Wei LuJohn J Schwarz
Jul 15, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Aruna PoduriAlan Daugherty
Oct 10, 2013·Circulation Research·Veronica SteriStephen Douglas Robinson
May 31, 2014·Circulation Research·Harita DharaneeswaranWilliam C Aird

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