PMID: 9428713Jan 15, 1998Paper

The contribution of water to the selectivity of pyruvate kinase for Na+ and K+

European Journal of Biochemistry
Leticia Ramírez-SilvaM Tuena de Gomez-Puyou

Abstract

For many years it has been known that K+ is an essential activator of pyruvate kinase [Kachmar, J. F. & Boyer, P. D. (1953) J. Biol. Chem. 200, 669-683] and that Na+ induces relatively small enhancements of activity. The effect of these two alkali metal ions on the activity of pyruvate kinase entrapped in the low water environment of reverse 'micelles formed with cetyltrimethylammonium, hexanol, n-octane and various water concentrations was studied. In reverse micelles with 3.6% water, the activity with 7 mM Na+ is more than 82 times higher than in aqueous solution with an equivalent Na+ concentration. As the concentration of water in reverse micelles is raised, the activating effect of relatively low concentrations of Na+ (or K+) decreases simultaneously to a more than 100-fold increase in the concentration of Na+ or K+ required for attaining half-maximal activation. Similar results were obtained with NH4+, Rb+ and Cs+. Therefore, the amount of water in the system is critical for observing activation by alkali metal ions. In fact, the concentration of Na+ required for half-maximal activation in standard aqueous media is higher than the concentrations that can be experimentally assayed. As evidenced from fluorescence and kineti...Continue Reading

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Citations

May 21, 2003·European Journal of Biochemistry·Leticia Ramírez-Silva, Jesús Oria-Hernández
Nov 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Swati PrasadEnrico Di Cera
Dec 5, 2014·International Journal of Molecular Sciences·Leticia Ramírez-SilvaNallely Cabrera
Sep 9, 2005·The Journal of Biological Chemistry·Jesús Oria-HernándezLeticia Ramírez-Silva
Aug 15, 2006·The Journal of Biological Chemistry·Jesús Oria-HernándezLeticia Ramírez-Sílva

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