PMID: 374998Jan 16, 1979Paper

The control region of the F sex factor DNA transfer cistrons: physical mapping by deletion analysis

Molecular & General Genetics : MGG
R Thompson, M Achtman

Abstract

A technique has been developed which allows the isolation of random deletions extending from unique restriction enzyme sites in plasmid DNA molecules. The method involves transformation of E. coli cells with linear plasmid DNAs generated by restriction enzyme cleavage. We have used this technique to map DNA transfer genes in the tra control region of F sex factor DNA. Deletions within EcoRI fragment f6 of F DNA have been isolated and used to assign physical locations to tra genes by a combination of genetic complementation tests, restriction enzyme analysis, DNA heteroduplexing and the analysis of the proteins synthesised in minicells and in vitro. Deletion analysis has also allowed the identification of the traK gene product.

References

Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J CarbonP Berg
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F HeffronS Falkow
Mar 7, 1977·Molecular & General Genetics : MGG·G Dougan, D Sherratt
Dec 15, 1977·Nature·N KennedyP Herrlich
Mar 1, 1978·Journal of Bacteriology·M AchtmanA J Clark
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K N TimmisM Achtman
Oct 24, 1978·Molecular & General Genetics : MGG·R Thompson, M Achtman
May 7, 1976·Molecular & General Genetics : MGG·C CoveyJ Carbon
Nov 1, 1976·CRC Critical Reviews in Biochemistry·R J Roberts
May 5, 1977·Journal of Molecular Biology·N Willetts
Aug 1, 1975·Journal of Bacteriology·M Achtman
Oct 23, 1975·Nature·R Helmuth, M Achtman
Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R A SkurrayA J Clark
Nov 1, 1972·Journal of Bacteriology·N S Willetts
Apr 1, 1973·Genetical Research·N S Willetts
Dec 31, 1973·Molecular & General Genetics : MGG·D Finnegan, N Willetts
Jan 1, 1971·Molecular & General Genetics : MGG·D J Finnegan, N S Willetts

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Citations

Apr 1, 1980·Molecular & General Genetics : MGG·S McIntire, N Willetts
Jan 1, 1981·Molecular & General Genetics : MGG·C PortierM Grumberg-Manago
Jan 1, 1984·Molecular & General Genetics : MGG·R C LundquistC I Kado
Jan 1, 1982·Molecular & General Genetics : MGG·R Thompson, L Taylor
May 1, 1993·Mutation Research·D Schulte-FrohlindeM Merz
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M AchtmanP Herrlich
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P A ManningM Achtman
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W Mandecki
Feb 11, 1982·Nucleic Acids Research·G J BoulnoisE Lanka
Nov 11, 1990·Nucleic Acids Research·P NorthJ Thacker
Mar 1, 1981·Plasmid·A M Brown, N S Willetts
Nov 1, 1982·Virology·E Van LeerdamP van de Putte
Jan 15, 1982·Journal of Molecular Biology·D J Galas, M Chandler
Mar 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·George T Lyozin, Luca Brunelli
Dec 1, 1982·Journal of Bacteriology·E C CornishJ Pittard
Apr 1, 1994·Journal of Bacteriology·S S PenfoldL S Frost
Apr 25, 1980·Journal of Molecular Biology·M AchtmanN Willetts
Nov 1, 1984·Plasmid·B P Dalrymple, P H Williams

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