The coordinate cellular response to insulin-like growth factor-I (IGF-I) and insulin-like growth factor-binding protein-2 (IGFBP-2) is regulated through vimentin binding to receptor tyrosine phosphatase β (RPTPβ).

The Journal of Biological Chemistry
Xinchun ShenDavid Clemmons

Abstract

Insulin-like growth factor-binding protein-2 (IGFBP-2) functions coordinately with IGF-I to stimulate cellular proliferation and differentiation. IGFBP-2 binds to receptor tyrosine phosphatase β (RPTPβ), and this binding in conjunction with IGF-I receptor stimulation induces RPTPβ polymerization leading to phosphatase and tensin homolog inactivation, AKT stimulation, and enhanced cell proliferation. To determine the mechanism by which RPTPβ polymerization is regulated, we analyzed the protein(s) that associated with RPTPβ in response to IGF-I and IGFBP-2 in vascular smooth muscle cells. Proteomic experiments revealed that IGF-I stimulated the intermediate filament protein vimentin to bind to RPTPβ, and knockdown of vimentin resulted in failure of IGFBP-2 and IGF-I to stimulate RPTPβ polymerization. Knockdown of IGFBP-2 or inhibition of IGF-IR tyrosine kinase disrupted vimentin/RPTPβ association. Vimentin binding to RPTPβ was mediated through vimentin serine phosphorylation. The serine threonine kinase PKCζ was recruited to vimentin in response to IGF-I and inhibition of PKCζ activation blocked these signaling events. A cell-permeable peptide that contained the vimentin phosphorylation site disrupted vimentin/RPTPβ association, ...Continue Reading

References

Nov 1, 1995·The Journal of Cell Biology·M OgawaraH Sugiura
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J I JonesD R Clemmons
Mar 14, 2000·Current Opinion in Cell Biology·S Dedhar
Dec 6, 2002·Endocrine Reviews·Sue M Firth, Robert C Baxter
May 22, 2007·Experimental Cell Research·Johanna IvaskaJohn E Eriksson
Mar 11, 2008·Journal of Proteome Research·Iveta DobrevaShoukat Dedhar
Mar 12, 2008·The Journal of Cell Biology·Hao-Ven WangReinhard Fässler
Dec 25, 2009·The EMBO Journal·Sara A WickströmReinhard Fässler
Feb 25, 2011·Molecular Biology of the Cell·Brian T HelfandRobert D Goldman
Oct 19, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Victoria E DeMambroDavid Clemmons
May 29, 2012·Journal of Cell Science·Moritz WidmaierReinhard Fässler
Jul 6, 2012·Current Opinion in Cell Biology·Jun Qin, Chuanyue Wu
Jul 19, 2013·Biochemical Society Transactions·Sushmita GhatakSara A Wickström
Apr 4, 2014·Biological & Pharmaceutical Bulletin·Chuanying XuYan Yu
May 20, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Gang XiDavid R Clemmons

❮ Previous
Next ❯

Citations

Apr 22, 2015·Molecular and Cellular Endocrinology·Jennifer A HetzMarilyn B Renfree
Dec 20, 2017·Journal of Molecular Endocrinology·L A Bach
Mar 23, 2018·Journal of Molecular Endocrinology·David R Clemmons
Jan 20, 2017·Journal of Molecular Endocrinology·Tingyuan RenJianquan Kan
Jan 17, 2016·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Gang XiDavid R Clemmons
May 6, 2021·Cell Reports Medicine·Justine FaramiaFrédéric Picard
May 8, 2021·Molecular Metabolism·Derek LeRoithBriony E Forbes
Jun 3, 2021·International Journal of Molecular Sciences·Julio SevillanoMaría Del Pilar Ramos-Álvarez

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.