The COP9 signalosome negatively regulates proteasome proteolytic function and is essential to transcription.

The International Journal of Biochemistry & Cell Biology
Huabo SuXuejun Wang

Abstract

The COP9 signalosome (CSN) is an evolutionarily conserved protein complex formed by eight subunits (CSN1 through CSN8). Deneddylating cullin family proteins is considered the bona fide function of the CSN. It has been proposed that the CSN regulates the assembly and disassembly of the cullin-based ubiquitin ligases via its deneddylation activity. Here we report that down-regulation of CSN8 by RNA interference destabilized differentially other CSN subunits and reduced the amount of CSN holo-complexes, leading to increases in neddylated cullin proteins and reduction of F-box protein Skp2 in HEK293 cells. Moreover, suppression of CSN8 enhanced the degradation of a proteasome surrogate substrate and cyclin kinase inhibitor p21(cip). Reduced transcript levels of cyclin kinase inhibitor p21(cip) and p27(kip) were also observed upon down-regulation of CSN8. These data suggest that the homeostatic level of CSN8/CSN suppresses proteasome proteolytic function and regulates transcription.

References

Oct 26, 1999·Current Biology : CB·S FreilichD A Chamovitz
Nov 13, 1999·Nature Cell Biology·A C CarranoM Pagano
Nov 13, 1999·Nature Cell Biology·H SutterlütyW Krek
May 5, 2001·Science·S LyapinaR J Deshaies
Nov 26, 2002·EMBO Reports·Vered Maytal-KivityMichael H Glickman
Mar 12, 2003·The EMBO Journal·Stefan UhleWolfgang Dubiel
May 6, 2003·The Journal of Biological Chemistry·Gil BornsteinAvram Hershko
May 10, 2003·Developmental Cell·Sergey DoronkinSteven K Beckendorf
Jun 14, 2003·Plant Physiology·Daniel A Chamovitz, Avital Yahalom
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Ning Wei, Xing Wang Deng
Apr 24, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Xin DongXuejun Wang
May 19, 2005·Molecular and Cellular Biology·Mathilde CalligéHélène Richard-Foy
Jul 28, 2005·The FEBS Journal·Xiaohua HuangWolfgang Dubiel
Sep 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Asangi R K KumarapeliXuejun Wang
Dec 24, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jinbao LiuXuejun Wang
Jun 20, 2006·European Journal of Cell Biology·Daniel Rosel, Alan R Kimmel
Sep 1, 2006·The Journal of Biological Chemistry·Simona DentiElisabetta Bianchi
Jan 26, 2007·Molecular Biology of the Cell·Zakir UllahR William Henry
Feb 24, 2007·The EMBO Journal·Katrin SchweitzerMichael Naumann

❮ Previous
Next ❯

Citations

Aug 22, 2009·Cardiovascular Research·Huabo Su, Xuejun Wang
Nov 26, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jie LiXuejun Wang
Nov 30, 2013·Cell Division·Shang-Nuan ZhangJun-Nian Zheng
Feb 19, 2013·Biochemical and Biophysical Research Communications·Gui-Ping XuJian-Hua Tong
Feb 14, 2012·Plant Science : an International Journal of Experimental Plant Biology·Johannes W Stratmann, Giuliana Gusmaroli
Jan 25, 2018·Scientific Reports·Ludwig GeistlingerLuiz Lehmann Coutinho
May 22, 2013·The Journal of Biological Chemistry·Cheng LiuNing Wei
Jun 15, 2019·Biomolecules·Jelena MilicJürgen Bernhagen
Nov 1, 2008·Circulation Research·Asangi R K KumarapeliXuejun Wang
Dec 10, 2019·American Journal of Physiology. Renal Physiology·Ryan J CorneliusDavid H Ellison
Oct 12, 2018·Journal of the American Society of Nephrology : JASN·Ryan J CorneliusDavid H Ellison

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Control & Proteolysis

Key regulators of cell cycle, including cyclins, cyclin dependent kinase inhibitors, DNA replication factors, are controlled by proteolysis. Discover the latest research on cell cycle control and proteolysis.