The copper influx transporter human copper transport protein 1 regulates the uptake of cisplatin in human ovarian carcinoma cells

Molecular Pharmacology
Alison K HolzerStephen B Howell

Abstract

Cells selected for resistance to cisplatin are often cross-resistant to copper and vice versa, and the major copper influx transporter copper transport protein 1 (CTR1) has been shown to regulate the uptake of cisplatin, carboplatin, and oxaliplatin in yeast. To further define the role of hCTR1 in human tumor cells, the ovarian carcinoma cell line A2780 was molecularly engineered to increase expression of hCTR1 by a factor of 20-fold. Enhanced expression of hCTR1 in the A2780/hCTR1 cells was associated with a 6.5-fold increase in basal steady-state copper content and a 13.7-fold increase in initial copper influx, demonstrating that the exogenously expressed hCTR1 was functional in altering copper homeostasis. The A2780/hCTR1 cells accumulated 46% more platinum after a 1-h exposure to 2 microM cisplatin, and 55% more after a 24 h exposure, than the control A2780/empty vector cells. The initial uptake of cisplatin was 81% higher in the A2780/hCTR1 cells when measured at 5 min. Thus, increased expression of hCTR1 had a substantially larger effect on the cellular pharmacology of copper than cisplatin. Interestingly, the increased uptake of copper and cisplatin was accompanied by only a marginal increase in sensitivity to the cytoto...Continue Reading

References

Apr 22, 1993·International Journal of Cancer. Journal International Du Cancer·A AtemaL A Smets
Jun 1, 1993·British Journal of Cancer·D P Gately, S B Howell
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·B Zhou, J Gitschier
Nov 5, 1997·Science·R A PufahlT V O'Halloran
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y M KuoJ Gitschier
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·J LeeD J Thiele
Jul 19, 2001·Cancer Chemotherapy and Pharmacology·E AmtmannG Schilling
Dec 6, 2001·The Journal of Biological Chemistry·Jaekwon LeeDennis J Thiele
May 25, 2002·The Biochemical Journal·Adriana E M KlompLeo W J Klomp
May 30, 2002·The Journal of Biological Chemistry·John F Eisses, Jack H Kaplan
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Seiko IshidaIra Herskowitz
Oct 23, 2002·Molecular Pharmacology·Xinjian LinStephen B Howell
Dec 27, 2002·The Journal of Biological Chemistry·Michael J PetrisDennis J Thiele
Oct 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Alison K HolzerStephen B Howell

❮ Previous
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Citations

Aug 10, 2013·European Journal of Clinical Pharmacology·Siddesh JaimanDeepa Khanna
Jul 27, 2005·Cancer Chemotherapy and Pharmacology·Shuichi KishimotoYoshikazu Takeuchi
Nov 11, 2008·Cancer Chemotherapy and Pharmacology·Cara A RabikM Eileen Dolan
Feb 24, 2007·Cancer Metastasis Reviews·Macus Tien KuoToshihisa Ishikawa
Jan 28, 2004·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Michael E FranksAlex Sparreboom
Mar 8, 2013·Hearing Research·Sofia Waissbluth, Sam J Daniel
Aug 8, 2013·Journal of the American Chemical Society·Oleg BondarevM Frederick Hawthorne
Dec 17, 2009·Nature Reviews. Clinical Oncology·Wim P Ceelen, Michael F Flessner
Mar 25, 2005·Nature Reviews. Drug Discovery·Dong Wang, Stephen J Lippard
Jan 5, 2010·Metallomics : Integrated Biometal Science·Ryan C Todd, Stephen J Lippard
Jun 27, 2008·Briefings in Functional Genomics & Proteomics·Mu WangDawn P G Brown
Apr 11, 2008·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·S B Howell
Nov 11, 2008·Molecular Pharmacology·Christopher A LarsonStephen B Howell
Feb 18, 2010·Molecular Pharmacology·Stephen B HowellMichael J Sailor
Apr 2, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Oleg Y Dmitriev
Oct 17, 2007·Annual Review of Pharmacology and Toxicology·Matthew D HallMichael M Gottesman
Sep 5, 2006·Cancer Research·Shuzhong ZhangKathleen M Giacomini
Jan 17, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Danielle D JandialStephen B Howell
Apr 16, 2013·The Journal of Clinical Investigation·Myrthala Moreno-SmithAnil K Sood
Nov 10, 2011·Toxins·Ronald P MillerWilliam Brian Reeves
Aug 14, 2012·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Yong ZhangXiao-xiao Zhang
Feb 15, 2012·Advances in Nutrition·Yanfang WangMichael J Petris
Jan 22, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Wissam BeainoCarolyn J Anderson
Feb 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stephen G Aller, Vinzenz M Unger
Jan 29, 2011·Metal-based Drugs·Paolo Abada, Stephen B Howell
Jan 11, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Christopher J De FeoVinzenz M Unger
Feb 21, 2013·Chemical Communications : Chem Comm·Alexis C Komor, Jacqueline K Barton
Jun 28, 2013·Metallomics : Integrated Biometal Science·Roohangiz SafaeiStephen B Howell
Oct 1, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sarah SpreckelmeyerAngela Casini
May 13, 2015·Molecular Medicine Reports·Sindhu Valiyaveedan GovindanAmritha Suresh
Aug 19, 2007·The American Journal of the Medical Sciences·Xin YaoKenneth Nugent
Feb 3, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Benny Zhitomirsky, Yehuda G Assaraf
Jul 17, 2012·Pharmacology & Therapeutics·Sandra M Sancho-MartínezFrancisco J López-Hernández
Mar 1, 2012·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Upendra Nadh KommuguriVijaya Lakshmi Bodiga
Jan 12, 2010·International Journal of Pharmaceutics·Michaela L KriegerGerd Bendas

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