PMID: 9420255Jan 7, 1998Paper

The coronavirus transmissible gastroenteritis virus causes infection after receptor-mediated endocytosis and acid-dependent fusion with an intracellular compartment

Journal of Virology
G H HansenO Norén

Abstract

Aminopeptidase N is a species-specific receptor for transmissible gastroenteritis virus (TGEV), which infects piglets, and for the 229E virus, which infects humans. It is not known whether these coronaviruses are endocytosed before fusion with a membrane of the target cell, causing a productive infection, or whether they fuse directly with the plasma membrane. We have studied the interaction between TGEV and a cell line (MDCK) stably expressing recombinant pig aminopeptidase N (pAPN). By electron microscopy and flow cytometry, TGEV was found to be associated with the plasma membrane after adsorption to the pAPN-MDCK cells. TGEV was also observed in endocytic pits and apical vesicles after 3 to 10 min of incubation at 38 degrees C. The number of pits and apical vesicles was increased by the TGEV incubation, indicating an increase in endocytosis. After 10 min of incubation, a distinct TGEV-pAPN-containing population of large intracellular vesicles, morphologically compatible with endosomes, was found. A higher density of pAPN receptors was observed in the pits beneath the virus particles than in the surrounding plasma membrane, indicating that TGEV recruits pAPN receptors before endocytosis. Ammonium chloride and bafilomycin A1 m...Continue Reading

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Citations

Jun 29, 2006·Cellular Microbiology·Cornelis A M de Haan, Peter J M Rottier
Aug 30, 2008·The Journal of General Virology·Evelien Van HammeHans J Nauwynck
Feb 6, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jian WangFei Liu
Aug 6, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gert H HansenE Michael Danielsen
May 3, 2015·Journal of Leukocyte Biology·Ileana Licona-LimónEnrique Ortega
May 1, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mallika GhoshLinda H Shapiro
Oct 6, 2020·Journal of Veterinary Science·Xiuli LiangWenju Zhang
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Oct 11, 2020·Vaccines·Nongluk Sriwilaijaroen, Yasuo Suzuki
Apr 11, 2006·The Journal of General Virology·Christine WinterGeorg Herrler

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