PMID: 701377Oct 1, 1978Paper

The corpus luteum of the guinea pig. II. Cytochemical studies on the Golgi complex, GERL, and lysosomes in luteal cells during maximal progesterone secretion

The Journal of Cell Biology
L G Paavola

Abstract

This study characterizes the cytochemical properties of the Golgi complex, the structure which corresponds to Golgi complex-endoplasmic reticulum-lysosomes (GERL), and the granule population in luteal cells of guinea pigs at the time of maximum progesterone secretion, in material fixed by vascular perfusion, a method particularly suited for preserving both fine structure and enzyme activity. The distribution of several marker enzymes was determined by electron microscope cytochemistry. Acid phosphatase (ACPase) and arylsulfatase were used to identify structures containing lysosomal proteins. To resolve specific problems, additional cytochemical markers were employed: localization of thiamine pyrophosphatase (TPPase) (in the Golgi complex) and alkaline phosphatase (ALPase) (a plasma membrane marker), and prolonged osmication (a generally accepted method of marking the outer cisterna of the Golgi complex). The results demonstrate that at the time of peak steroid secretion the Golgi complex in luteal cells, in marked contrast to that of most other cell types, typically displays intense ACPase activity in all of its cisternae. Similarly, all Golgi cisternae stain after prolonged osmication and may show TPPase activity. On the other...Continue Reading

References

Aug 28, 1975·Histochemistry·M Borgers, F Thoné
Jun 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S GoldfischerB Schiller
Apr 1, 1969·The Journal of Cell Biology·D S Friend
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J L Ericsson
Jan 1, 1974·The Journal of Cell Biology·M G FarquharG E Palade
Apr 1, 1973·The Journal of Cell Biology·R W Young
Sep 1, 1973·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Borgers
Jun 1, 1971·The Journal of Cell Biology·R D CheethamD S Friend
Jan 1, 1970·Zeitschrift für Zellforschung und mikroskopische Anatomie·L BjersingR V Short
May 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Hugon, M Borgers
Jan 1, 1967·The Journal of Cell Biology·B Bertolini, G Hassan
Jul 1, 1965·The American Journal of Anatomy·D S FRIEND, M J MURRAY

❮ Previous
Next ❯

Citations

Jan 1, 1991·Journal of Electron Microscopy Technique·M Pavelka, A Ellinger
Jan 1, 1992·Tissue & Cell·A T Bijovsky, P A Abrahamsohn
May 1, 1985·Journal of Ultrastructure Research·M C MagalhãesM M Magalhães
Feb 1, 1989·The Journal of Cell Biology·G GriffithsK Simons
Jan 1, 1990·Progress in Histochemistry and Cytochemistry·C E SmithJ J Bergeron
Jan 1, 1983·Alcoholism, Clinical and Experimental Research·M A Tavares, M M Paula-Barbosa
Apr 1, 1982·The American Journal of Anatomy·X M TangY Clermont
Jul 15, 1999·Nature Biotechnology·A J LinkJ R Yates
Mar 1, 1987·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·I I SingerJ W Huff
Oct 5, 1987·Biochimica Et Biophysica Acta·J Roth

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.